Factor XIII

Factor XIII crosslinks fibrin

coagulation factor XIII, A1 polypeptide Identifiers Symbol F13A1 Alt. Symbols F13A Entrez 2162 HUGO 3531 OMIM 134570 RefSeq NM_000129 UniProt P00488 Other data Locus Chr. 6 p24.2-p23

coagulation factor XIII, B polypeptide Identifiers Symbol F13B Entrez 2165 HUGO 3534 OMIM 134580 RefSeq NM_001994 UniProt P05160 Other data Locus Chr. 1 q31-q32.1

Factor XIII or fibrin stabilizing factor is an enzyme (EC 2.3.2.13) of the blood coagulation system that crosslinks fibrin.

Function

Factor XIII is a transglutaminase that circulates in the plasma as a heterotetramer of two catalytic A subunits and two carrier B subunits. When thrombin has converted fibrinogen to fibrin, the latter forms a proteinaceous network in which every E-unit is crosslinked to only one D-unit. Factor XIII is activated by thrombin into factor XIIIa; its activation into Factor XIIIa requires calcium as a cofactor. A cleavage by thrombin between residue Arg37 and Gly38 on the N-terminus of the A subunit, leads to the release of the activation peptide (MW 4000 da). In the presence of calcium the carrier subunits dissociate from the catalytic subunits, leading to a 3D change in conformation of factor XIII and hence the exposure of catalytic cysteine residue. Upon activation by thrombin, factor XIIIa acts on fibrin to form γ-glutamyl-Є-lysyl amide cross links between fibrin molecules to form an insoluble clot.

Discovery

FXIII is known also as Laki-Lorand factor, after the scientists who first proposed its existence in 1948.^[1] A 2005 conference recommended standardization of nomenclature.^[2]

Genetics

Zymogen factor XIII is a 320000 M_r glycoprotein tetramer consisting of twice two subunits (2 A and 2 B),^[2] the genes for which are on different chromosomes:

• A subunit (6p25-p24). The transglutaminase part; this adds an alkyl group to the nitrogen on a glutamine residue, which binds in turn with a lysine on the other chain. The molecular weight of the A chain is approximately 83000.
• B subunit (1q31-q32.1). This has no clear enzymatic activity, and may serve as a carrier for the A subunit. The molecular weight of the B chain is approximately 76500.

Physiology

Typical concentrations of FXIII in plasma is 10 μg/ml (2A2B heterodimer), while the concentration of free B chain is 22 μg/ml. FXIII has a long half life, ranging from 5-9 days. It is present in plasma, platelets, and monocytes, as well as macrophages and bone marrow precursors of these cell types.^[2]

A clot that has not been stabilized by FXIIIa is soluble in 5 mol/L urea, while a stabilized clot is resistant to this phenomenon.^[1]

Diagnostic use

Factor XIII levels are not measured routinely, but may be considered in patients with an unexplained bleeding tendency. As the enzyme is quite specific for monocytes and macrophages, determination of the presence of factor XIII may be used to identify and classify malignant diseases involving these cells.^[2]

See also

• Factor XIII deficiency
• D-dimer

References

External links

• Factor XIII deficiency at hemophilia.org
• med/3491 at eMedicine