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Q: Video or animation of gel filtration chromatography?

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Filtration separates particles based on size.

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in gel filtration the volume of eluent is what moves compounds in and out of the pores in the matrix.A large volume of sample means the sample components will not be introduced to the matrix as a tight "plug" and the sample will slowly spread into the matrix meaning the beginning and the ending of the sample will be far apart meaning the sample will be spread out over the gel matrix resulting in poor resolution of that component from any other.

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