Usually DNA is obtained from bacterial cultures. To break the bacterial cell wall, there are several options (e.g., sonications, change in atmospheric pressure, etc.), however, there is another and milder method that many molecular biologists prefer in order to protect the DNA content the more possible, especially when the DNA that is wanted is related to plasmids. To do this, researchers uses the enzyme lysozyme in a coctail to digest cell walls, because lysozyme catalyzes the hydrolysis of polysaccharides that occur in the glycopeptide layer of bacterial cell walls. After lysozyme digestion, a mild detergent is added (e.g., SDS) to finish the cell wall breakdown.