Glycosylation

Glycosylation (see also chemical glycosylation) is the reaction in which a carbohydrate, i.e. a glycosyl donor, is attached to a hydroxyl or other functional group of another molecule (a glycosyl acceptor). In biology glycosylation refers to the enzymatic process that attaches glycans to proteins, lipids, or other organic molecules. This enzymatic process produces one of the fundamental biopolymers found in cells (along with DNA, RNA, and proteins). Glycosylation is a form of co-translational and post-translational modification. Glycans serve a variety of structural and functional roles in membrane and secreted proteins.^[1] The majority of proteins synthesized in the rough ER undergo glycosylation. It is an enzyme-directed site-specific process, as opposed to the non-enzymatic chemical reaction of glycation. Glycosylation is also present in the cytoplasm and nucleus as the O-GlcNAc modification. Five classes of glycans are produced:

• N-linked glycans attached to a nitrogen of asparagine or arginine side-chains
• O-linked glycans attached to the hydroxy oxygen of serine, threonine, tyrosine, hydroxylysine, or hydroxyproline side-chains, or to oxygens on lipids such as ceramide
• phospho-glycans linked through the phosphate of a phospho-serine;
• C-linked glycans, a rare form of glycosylation where a sugar is added to a carbon on a tryptophan side-chain
• glypiation, which is the addition of a GPI anchor that links proteins to lipids through glycan linkages.

Purpose

The carbohydrate chains attached to the target proteins serve various functions.^[2] For instance, some proteins do not fold correctly unless they are glycosylated first.^[1] Also, polysaccharides linked at the amide nitrogen of asparagine in the protein confer stability on some secreted glycoproteins. Experiments have shown that glycosylation in this case is not a strict requirement for proper folding, but the unglycosylated protein degrades quickly. Glycosylation may play a role in cell-cell adhesion (a mechanism employed by cells of the immune system), as well.

Mechanisms

There are various mechanisms for glycosylation, although most share several common features:^[1]

• Glycosylation, unlike glycation, is an enzymatic process
• The donor molecule is often an activated nucleotide sugar
• The process is site-specific.

Types of glycosylation

N-linked glycosylation

N-linked glycosylation is important for the folding of some eukaryotic proteins. The N-linked glycosylation process occurs in eukaryotes in the lumen of the endoplasmic reticulum and widely in archaea, but very rarely in bacteria.

O-linked glycosylation

O-linked glycosylation is a form of glycosylation that occurs in the Golgi apparatus.^[3]

Phospho-serine glycosylation

Xylose, fucose, mannose, and GlcNAc phospho-serine glycans have been reported in the literature. Fucose and GlcNAc have been found only in Dictyostelium discoideum, mannose in Leishmania mexicana, and xylose in Trypanosoma cruzi. Mannose has recently been reported in a vertebrate, the mouse, Mus musculus, on the cell-surface laminin receptor alpha dystroglycan⁴. It has been suggested this rare finding may be linked to the fact that alpha dystroglycan is highly conserved from lower vertebrates to mammals.^[4]

C-mannosylation

A mannose sugar is added to the first tryptophan residue in the sequence W-X-X-W (W indicates tryptophan; X is any amino acid). Thrombospondins are one of the most commonly C-modified proteins, although this form of glycosylation appears elsewhere as well. C-mannosylation is unusual because the sugar is linked to a carbon rather than a reactive atom such as nitrogen or oxygen. Recently, the first crystal structure of a protein containing this type of glycosylation has been determined - that of human complement component 8, PDB ID 3OJY.

Formation of GPI anchors (glypiation)

A special form of glycosylation is the formation of a GPI anchor. In this kind of glycosylation a protein is attached to a lipid anchor, via a glycan chain. (See also prenylation.)

See also

• Glycorandomization
• Glycation
• Advanced glycation endproduct
• Chemical glycosylation
• Fucosylation

References

External links

• Online textbook of glycobiology with chapters about glycosylation
• GlyProt: In-silico N-glycosylation of proteins on the web
• NetNGlyc: The NetNglyc server predicts N-glycosylation sites in human proteins using artificial neural networks that examine the sequence context of Asn-Xaa-Ser/Thr sequons.
• Supplementary Material of the Book "The Sugar Code"