It is the enzyme that causes the oxidation of glycolic acid [glycolate] {HOCH2COOH} to glyoxylic acid {OHCCOOH}.

The amino acid responsible for the Hopkins-Cole reaction is the tryptophan because of its indole ring that in the reaction forms a violet color upon treatment of the sample with glyoxylic acid and sulfuric acid.

H2O is Dihydrogen Monoxide, which is water.

CO is Carbon Monoxide, which is a harmful gas produced by incomplete combustion of hydrocarbons. For example, the burning of gasoline in a car.

Also it would be buring of a oil

Poisoning from antifreeze which today is usually ethylene glycol -- a clear, colorless, odorless liquid with a sweet taste -- that can produce dramatic and dangerous toxicity.

Many cases of ethylene glycol poisoning are due to the ingestion of it by children. They may take in large amounts since the substance tastes good. Alcoholics may also drink it as a substitute for alcohol (ethanol).

Ethylene glycol is itself relatively nontoxic. However, it is metabolized (changed) in the body to glycolic acid, glyoxylic acid and oxalic acid, which are highly toxic compounds.

There can be widespread tissue injury in the kidney, brain, liver, and blood vessels. The result can be fatal.

This is serious and must be treated by a doctor immediately.

Sulfuric acid, Hydrochloric acid, Nitric acid, Phosphoric Acid