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1. Why is the phosphate buffer made up by using the Henderson-Hasselbalch equation not the expected pH?

Q: Why is the phosphate buffer made up by using the Henderson hasselbalch equation not the expected PH?

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1. Why is the phosphate buffer made up by using the Henderson-Hasselbalch equation not the expected pH?

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The Henderson-Hasselbach equation provides a general solution to the quantitative treatment of acid-base equilibria in biological systems (Garrett & Grisham, 2005, Biochemistrym, p.42).

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Its an equation you can use to find the pH of a solution.

it is.... --- pH = pKa + log (Base/Acid) ---

these may help too

Ka = 10^-pKa

Kw = Ka*Kb

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the pH of ethanol can be calculated using its pKa value (pKa 15.9) and the Henderson-Hasselbalch equation.

pH = pKa - log [AH/A]

where [AH/A] the ratio of disassociated versus undisassociated species in solution.

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pH = pKa + log [sodium acetate]/[acetic acid] = Henderson Hasselbalch equation

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