| Sci-Tech Dictionary: historadiography |
(biophysics) A technique for taking x-ray pictures of cells, tissues, or sometimes whole small organisms.
Historadiography
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| Sci-Tech Encyclopedia: Historadiography |
The technique for taking x-ray pictures of cells, tissues, or sometimes the whole animal or plant, if it is a small one. Soft x-rays, those with low penetrating power and relatively long wavelengths, are required for this type of picture. The best pictures are obtained when the tissues contain deposits of metallic elements which have a high absorption capacity for x-rays. See also X-rays.
In applying the technique to tissues, a relatively thin section is placed against an x-ray film and irradiated with a beam of x-rays. When the film is developed, a picture of the object or section of tissue shows on the film. Another method attempts to focus the x-rays after they pass through the specimen. See also X-ray microscope.
| Wikipedia: Historadiography |
Historadiography is a technique formerly utilized in the fields of histology and cellular biology to provide semiquantitative information regarding the density of a tissue sample. It is usually synonymous with microradiography.[1] This is achieved by layering a ground section of mineralized tissue (such as bone) with photographic emulsion on a glass slide and exposing the sample to a beam of X-rays. After developing the emulsion, the resulting radiograph can be viewed with a microscope. A side-by-side comparison with a slide containing radiographs of various substances of known mass can provide a rough mass estimate, and therefore a rough approximation of the concentration of calcium salts in the sample.
Historadiography has also been used to visualize staining of tissue, such as spinal cord samples with thorotrast, which contains thorium that is opaque to X-rays.[2]
Over recent decades researchers have generally lost interest in historadiography. The most recent publication using the term (1998) to be indexed in PubMed[3] referred to autoradiography of tritium incorporated in thymidine.[4]
References
- ^ "CancerWeb Online Medical Dictionary". http://cancerweb.ncl.ac.uk/cgi-bin/omd?historadiography.
- ^ F. P. BOHATIRCHUK , T. F. JELETZKY , and L. P. IVAN (1973). "CONTACT HISTORADIOGRAPHY OF NERVOUS TISSUE IMPREGNATED WITH THOROTRAST". http://www.ajronline.org/cgi/content/abstract/118/4/923.
- ^ "PubMed search: historadiography". http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=historadiography.
- ^ PMID 9555564
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 | X-rays (atomic and molecular physics) |
| Autoradiograph |
| Thorotrast |
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