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The short story is titled "Sally" by Isaac Asimov. It revolves around a robot named QT-1 who is trained to compose music. When the robot's owner is moved to tears by the music, the robot interprets the tears as a sign of failure and decides never to play music again.

the robot chicken theme song

This is kinda tough.

Put on the Green Splatter hat or the Black Music Hat.

Put on the Green or Blue Music Shirt (with baggy white sleeves).

Dance. You do the robot!

The title of the short story is "The Music Master." It tells the tale of a robot who learns to play music flawlessly but stops performing when he realizes his music causes his master to cry.