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the main reason to do immunodiffusion test is to quantify antigen or antibody

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the main reason to do immunodiffusion test is to quantify antigen or antibody

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SDS-PAGE method

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an electric current is passed through the agar in electroimmunodiffusion assays and therefore separates antigens based on their isoelectric point. Immunodiffusion does not involve electrophoresis and can be used to assess Ab to Ag binding through the visualization of a precipitate line

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Because G. Mancini is the name of the scientist who invented the method. http://www.sciencedirect.com/science/article/pii/0019279165900042.

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1.used for determining the antigen concentration of unknown sample.
2.to identify the specific viral antigen from the mixed viral sample.
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