Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye such as fluorescein isothiocyanate (FITC). There are two major types of immunofluorescence staining methods: 1) direct immunofluorescence staining in which the primary antibody is labeled with fluorescence dye, and 2) indirect immunofluorescence staining in which a secondary antibody labeled with fluorochrome is used to recognize a primary antibody. Immunofluorescence staining can be performed on cells fixed on slides and tissue sections. Immunofluorescence stained samples are examined under a fluorescence microscope or confocal microscope

immunofluorescence applied to identify a number of subpopulatons of lymphocytes

suitable for identify bacterial diseases

detecting complement component tissue and other cellular product in situ

is used to localization of the antigen in tissue section or sub cellular compartmant

Immunofluorescence

true

The DIF (direct immunofluorescence) test on a skin biopsy involves staining the tissue sample with fluorescently labeled antibodies to detect the presence and localization of specific proteins (such as antibodies or immune complexes) in the skin. This test helps in diagnosing autoimmune skin conditions such as pemphigus vulgaris, bullous pemphigoid, and lupus erythematosus.