Inoculum

Inoculum

temperature, initial cellular mean mass, maintenance energy, effects of inoculum size, inoculum size and mean generation times

The purpose of heating the inoculating materials before and after using them is for sterilization. They must be sterilized before to kill any bacteria already on them so that they do not contaminate anything during use, and they must be sterilized after to get off the bacteria contacted from use.

purity of culture, depth of seeded layer, incubation temp, agar temp, size of inoculum, distribution of inoculum, incubation period, diffusion rate of antibiotic, concentration of antibiotic on disk, growth rate of bacterium.