They all try to kill James Bond.

Pussy Galore

There were absolutely loads of villains in the James Bond films so I will only provide you with the most notorious of the villains. Ernst Stavro Blofeld who was the head of the secret terrorist organisation known as S.P.E.C.T.R.E. Out of all the Bond villains Blofled is probably considered the most evil of them all. Some of the characteristics of this criminal mastermind are his brilliant criminal mind, his ruthlessness, and his snobbishness. He appeared in the James Bond films; From Russia With Love (1963), Thunderball (1965), You Only Live Twice (1967), On Her Majesty's Secret Service (1969), Diamonds Are Forever (1971), and then in For Your Eyes Only (1981). Meaning that Ernst Stavro Blofeld is the most notorious of all the villains.

In the 1995 James Bond film GoldenEye, villains Alec Trevelyan and Xenia Onatopp escape from a Soviet-era train by flying away in a helicopter.

I believe only men have acted as James Bond.