To determine the commonly deleted region on chromosome
3 in carcinoma of the uterine cervix, these samples were
also
examined for loss of heterozygosity at 5 other loci on chro
mosome 3: RAFl(3p24-25) (14); ERBAß(3p22-24.l ) (17);
DNF15S2 (3p21) (13); D3S3(3pl4) (13); and SST(3q28) (13).
Loss of heterozygosity at the RAF1 locus and the ERBAßlocus
was observed in one of 3 patients and 3 of 7 patients (Fig. 1,
b
c), respectively, but no loss was observed at 2 other loci,
DNFI5S2 and SST, whereas duplication of one of two alÃeles
was observed in 2 of 6 patients at SST (Table 1). Analysis
of
loss of heterozygosity at the ERBAßlocus was performed
using
2 different DNA probes: a human ERBAßcomplementary DNA
clone, pheA4; and a genomic ERBAßDNA clone, pBH302 (15-
17). The pheA4 and pBH302 probes detect BamHl and Hindlll
RFLP, respectively, and loss of heterozygosity at this locus
was
observed in none of 2 patients by using the pheA4 probe and
in 3 of 5 patients by the pBH302 probe (Table 1; Fig.
lé).No
information was available on loss of heterozygosity at the
D3S3
locus, because none of the 18 patients was heterozygous in
normal tissue at this locus. Therefore, the commonly deleted
chromosomal region in carcinoma of the uterine cervix is
probably a small part of the short arm of chromosome 3,
including the D3S2 locus. These results strongly suggest
that
recessive genetic changes on chromosome 3p are involved in
the development of carcinoma of the uterine cervix.
