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o-nitroaniline is eluted first due to the polarity of it and the silica

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o-nitroaniline is eluted first due to the polarity of it and the silica

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The mixture of o-Nitroaniline and P-Nitroaniline can be separated using liquid chromatography because they have close solubility.

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It reacts with the acidic conditions (since the NH2 is basic) to form the meta-directing electron withdrawing group NH3. This forms the meta-nitroaniline.

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Quinten Denman

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m-Nitroaniline

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