The phenylhydrazine test is also known as the Osazone test. The
phenylhydrazine test is used for reducing the amounts of
carbohydrates, momosacchstides, and disaccharides.
The phenylhydrazine test is also known as the Osazone test. The
phenylhydrazine test is used for reducing the amounts of
carbohydrates, momosacchstides, and disaccharides.
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water
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Reagents of Osazone test are 1. Phenylhydrazine mixture
consisting of phenyl-hydrazine and anhydrous sodium citrate 2.
Orignal solution containing a carbohydrate
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After correcting the spelling of the Question Here is the answer for it.
Osazones are a class of carbohydrate derivatives formed when sugars are reacted with phenylhydrazine. The reaction involves the formation of a pair of phenylhydrazone functionalities, concomitant with the oxidation of the hydroxymethylene group adjacent to the formyl center. The reaction can be used to identify monosaccharides. It involves two reactions. Firstly glucose with phenylhydrazine gives us glucosephenylhydrazone by the elimination of a water molecule from the functional group.The next step involves reaction of one mole of glucosephenylhydrazone with two moles of phenylhydrazine (excess). First, phenylhydrazine is involved in oxidizing the alpha carbon to a carbonyl group, and the second phenylhydrazine involves removal of one water molecule with the formyl group of that oxidized carbon and forming the similar carbon-nitrogen bond. The alpha carbon is attacked here because it is more reactive than the others.They are highly colored and crystalline compounds and can be easily detected. Glucose gives broomstick shaped crystals with this whereas maltose gives sunflower shaped crystals.
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Mariam Mashregi has written:
'Accelerated cytotoxic mechanism screening of isoniazid and
phenylhydrazine using an in vitro hepatocyte inflammation
model'