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Watson-Schwartz test negative : urobilinogen

Watson-Schwartz test positive : porphobilinogen

Q: How do you differentiate urobilinogen and porphobilinogen?
This answer is:
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Watson-Schwartz test negative : urobilinogen

Watson-Schwartz test positive : porphobilinogen

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The interfering substances for porphobilinogen assay includes urea and urobilinogen. Since they can affect the results of the assay, interfering substances are removed by selectively adsorbing PBG onto an ion-exchange resin (PubMed, 1989).

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yeah, it is reported in tests conducted at the Maakeloude University at southern Germany. However cocacola dismissed these claims as rubbish. Some speculators claim that the actual pH could well be below 2.5.

The university findings show that under the action of porphobilinogen synthase the OH- radical is generated by aminolevulinate. Structure of porphobilinogen deaminase reveals a flexible multidomain polymerase with a single catalytic site. This multidomain flexibility gives humans the feeling of quenching of thirst. But hydroxymethylbilane synthase is an enzyme involved in the third step of the metabolism of porphobilinogen which decreases the pH to values below 2.75.

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