Purkinje cell

Neuron: Purkinje cell
Drawing of pigeon Purkinje cells (A) by Santiago Ramon y Cajal
Location	Cerebellum
Function	inhibitory projection neuron
Neurotransmitter	GABA
Morphology	flat dendritc arbor
Presynaptic connections	Parallel fibers and Climbing fibers
Postsynaptic connections	Cerebellar deep nuclei
NeuroLex ID	sao471801888

For the cells of the electrical conduction system of the heart, see Purkinje fibers

Purkinje cells, or Purkinje neurons (pronounced /pərˈkɪndʒi/ purr-KIN-jee), are a class of GABAergic neurons located in the cerebellar cortex. They are named after their discoverer, Czech anatomist Jan Evangelista Purkyně (Czech: [ˈpurkɪɲɛ]).

Anatomy

Transverse section of a cerebellar folium. (Purkinje Cell labeled at center top.)

These cells are some of the largest neurons in the human brain (Betz cells being the absolute largest)^[1], with an intricately elaborate dendritic arbor, characterized by a large number of dendritic spines. Purkinje cells are found within the Purkinje layer in the cerebellum. Purkinje cells are aligned like dominos stacked one in front of the other. Their large dendritic arbors form nearly two-dimensional layers through which parallel fibers from the deeper-layers pass. These parallel fibers make relatively weaker excitatory (glutamatergic) synapses to spines in the Purkinje cell dendrite, whereas climbing fibers originating from the inferior olivary nucleus in the medulla provide very powerful excitatory input to the proximal dendrites and cell soma. Parallel fibers pass orthogonally through the Purkinje neuron's dendritic arbor, with up to 200,000 parallel fibers forming a synapse with a single Purkinje cell. Each Purkinje cell receives a synapse from only a single climbing fiber. Both basket and stellate cells (found in the cerebellar molecular layer) provide inhibitory (GABAergic) input to the Purkinje cell, with basket cells synapsing on the Purkinje cell axon initial segment and stellate cells onto the dendrites.

Purkinje cells send inhibitory projections to the deep cerebellar nuclei, and constitute the sole output of all motor coordination in the cerebellar cortex.

Electrophysiological activity

Microcircuitry of the cerebellum. Excitatory synapses are denoted by (+) and inhibitory synapses by (-).
MF: Mossy fiber.
DCN: Deep cerebellar nuclei.
IO: Inferior olive.
CF: Climbing fiber.
GC: Granule cell.
PF: Parallel fiber.
PC: Purkinje cell.
GgC: Golgi cell.
SC: Stellate cell.
BC: Basket cell.

Purkinje cells show two distinct forms of electrophysiological activity:

• Simple spikes occur at rates of 17 – 150 Hz (Raman and Bean, 1999) either spontaneously or and when Purkinje cells are activated synaptically by the parallel fibers, the axons of the granule cells.

• Complex spikes are rapid (>300 Hz) bursts of spikes caused by climbing fiber activation, and can involve the generation of calcium-mediated action potentials in the dendrites. Following complex spike activity simple spikes can be suppressed by the powerful complex spike input.

Purkinje cells show spontaneous electrophysiological activity in the form of trains of spikes both sodium- as well as calcium-dependent was initially shown by Rodolfo Llinas (Llinas and Hess (1977) and Llinas and Sugimori (1980). P-type calcium channels were named after Purkinje cells where they were initially encountered (Llinas et al. 1989), which are crucial in cerebellar function. It has recently been shown that climbing fiber activation of the Purkinje cell can shift its activity from a quiet state to a spontaneously-active state, and vice-versa, serving as a type of toggle switch (Lowenstein et al., 2005, Nature Neuroscience). However, these findings have recently been challenged by a study suggesting that such toggling by climbing fiber inputs occurs predominantly in anaesthetized animals, and that Purkinje cells in awake behaving animals, in general, operate almost continuously in the upstate (Schonewille et al., 2006, Nature Neuroscience).

Findings have suggested that Purkinje cell dendrites release endocannabinoids that can transiently downregulate both excitatory and inhibitory synapses^[2]

Medical conditions related to Purkinje cells

In humans, Purkinje cells are affected in a variety of diseases ranging from toxic exposure (alcohol, lithium), to autoimmune diseases and to genetic mutations (spinocerebellar ataxias, Unverricht-Lundborg disease, autism) and neurodegenerative diseases that are not thought to have a known genetic basis (cerebellar type of multiple system atrophy, sporadic ataxias).

In some domestic animals, a condition where the Purkinje cells begin to atrophy shortly after birth, called Cerebellar abiotrophy, can lead to symptoms including ataxia, intention tremors, hyperreactivity, lack of menace reflex, stiff or high-stepping gait, apparent lack of awareness of wherein the feet are (sometimes standing or walking with a foot knuckled over), and a general inability to determine space and distance.^{[citation needed]} A similar condition known as cerebellar hypoplasia occurs when Purkinje cells either fail to develop in utero or die off in utero prior to birth. Ataxia Telangiectasia and Niemann Pick disease Type C are a genetic conditions, in which Purkinje cells are progressively lost.

Links

• Cell Centered Database - Purkinje
• Cerebellar disorders
• Disorders of cerebellum
• NIF Search - Purkinje Cell via the Neuroscience Information Framework

References

• Llinás R, Hess R (July 1976). "Tetrodotoxin-resistant dendritic spikes in avian Purkinje cells". Proc. Natl. Acad. Sci. U.S.A. 73 (7): 2520–3. doi:10.1073/pnas.73.7.2520. PMID 1065905. 
• Llinás R, Sugimori M (August 1980). "Electrophysiological properties of in vitro Purkinje cell somata in mammalian cerebellar slices". J. Physiol. (Lond.) 305: 171–95. PMID 7441552. PMC: 1282966. http://www.jphysiol.org/cgi/pmidlookup?view=long&pmid=7441552. 
• Llinás RR, Sugimori M, Cherksey B (1989). "Voltage-dependent calcium conductances in mammalian neurons. The P channel". Ann. N. Y. Acad. Sci. 560: 103–11. doi:10.1111/j.1749-6632.1989.tb24084.x. PMID 2545128. 

1. ^ Purves, Dale, George J. Augustine, David Fitzpatrick, William C. Hall, Anthony-Samuel LaMantia, James O. McNamara, and Leonard E. White (2008). Neuroscience. 4th ed.. Sinauer Associates. pp. 432–4. ISBN 978-0-87893-697-7. 
2. ^ Kreitzer AC, Regehr WG (March 2001). "Retrograde inhibition of presynaptic calcium influx by endogenous cannabinoids at excitatory synapses onto Purkinje cells". Neuron 29 (3): 717–27. doi:10.1016/S0896-6273(01)00246-X. PMID 11301030.