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puromycin

 
Dictionary: pu·ro·my·cin
(pyʊr'ə-mī'sĭn) pronunciation
n.
An antibiotic, C22H29N7O5, obtained from the soil bacterium Streptomyces alboniger, that is used experimentally as an inhibitor of protein synthesis.

[PUR(INE) + -MYCIN.]


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Veterinary Dictionary: puromycin
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An antibiotic that inhibits protein synthesis. Used in the treatment of protozoal infections and as an antineoplastic agent.

Wikipedia: Puromycin
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Puromycin
Puromycin.svg
IUPAC name
Identifiers
CAS number 53-79-2
PubChem 5891
MeSH Puromycin
SMILES
Properties
Molecular formula C22H29N7O5
Molar mass 471.51 g mol−1
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

Puromycin is an antibiotic that is a protein synthesis inhibitor by inhibiting prokaryotic translation.

Contents

Inhibition of translation

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium,[1] that causes premature chain termination during translation taking place in the ribosome. Part of the molecule resembles the 3' end of the aminoacylated tRNA. It enters the A site and transfers to the growing chain, causing premature chain release. The exact mechanism of action is unknown at this time, but, the 3' position contains an amide linkage instead of the normal ester linkage of tRNA, the amide bond makes the molecule much more resistant to hydrolysis and thus causes the ribosome to become stopped.

It is not selective for either prokaryotes or eukaryotes.

Also of note, puromycin is critical in mRNA display as it allows the growing peptide chain to be covalently bonded to its own mRNA template.

Cell culture

Puromycin is used in cell biology as selective agent in cell culture systems. It is toxic to prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain. Puromycin is soluble in water (50mg/ml) as colorless solution at 10 mg/ml. Puromycin is stable for one year as solution when stored at -20°C. The recommended dose as a selection agent in cell cultures is within a range of 1-10 μg/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/ml. It acts quickly and can kill up to 99% of nonresistant cells within 2 days.


Selection of Escherichia coli

Puromycin is poorly active on E. coli. Puromycin-resistant transformants are selected in LB agar medium supplemented with 125 µg/ml of puromycin. But use of puromycin for E. coli selection requires precise pH adjustment and also depends on which strain is selected. For hassle–free selection and optimum results the use of special modified puromycin is possible. Plates containing puromycin are stable for 1 month when stored at 4°C.

References




 
 
Learn More
Gene Targeting
O-demethylpuromycin O-methyltransferase
NPEPPS

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Copyrights:

Dictionary. The American Heritage® Dictionary of the English Language, Fourth Edition Copyright © 2007, 2000 by Houghton Mifflin Company. Updated in 2009. Published by Houghton Mifflin Company. All rights reserved.  Read more
Veterinary Dictionary. Saunders Comprehensive Veterinary Dictionary 3rd Edition. Copyright © 2007 by D.C. Blood, V.P. Studdert and C.C. Gay, Elsevier. All rights reserved.  Read more
Wikipedia. This article is licensed under the Creative Commons Attribution/Share-Alike License. It uses material from the Wikipedia article "Puromycin" Read more