The spectrofluorometer is an instrument which takes one advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample. A certain excitation wavelength is selected, and the emission is observed either at a single wavelength or a scan is performed to record the intensity versus wavelength also called an emission spectra

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They both measure fluorescence, but a spectrofluorometer usually has adjustable excitation and emission wavelengths via a monochromator; whereas a fluorometer usually measures fixed wavelengths either by filters, monochromators or fixed diodes. Spectrofluorometers are more flexible but fluorometers are much more sensitive, usually 100-1000x more sensitive.

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