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how to prepare citrate buffer for making Streptozotocin

Q: Is citrate buffer sterile for making Streptozotocin?

This answer is:

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because the alloxan is cheaper than the streptozotocin..

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Michael Costigan has written:

'Basement membrane gene expression in the normal and streptozotocin diabetic rat'

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Animal Models of Diabetic Complications Consortium AMDCC Protocols Low-Dose Streptozotocin Induction Protocol (Mouse)

Version: 1

Replaced by version: N/A

Edited by: The University of Michigan Medical Center , Frank Brosius

Summary

Reagents and Materials

Protocol

Reagent Preparation

Na-Citrate Buffer

Streptozotocin (STZ)

STZ-Na-Citrate Solution

Summary: This protocol is used by AMDCC members to induce diabetes in a number of the animal models developed by the consortium. This induction protocol is used to induce a diabetes similar to a type I diabetic.

Reagents and Materials: Reagent/Material

Quantity Required

Vendor

Stock Number

STZ-Na-Citrate Solution

See below

TB or insulin syringes w/ needle attached (26-28 Â½ gauge)

As many as necessary for the number of mice

Isoflurane drop jar

Isoflurane

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I am not sure whether you have read this already. Anyways, the Effect of Cassia Auriculata Flowers on Blood Sugar Levels, Serum and Tissue Lipids in Streptozotocin Diabetic Rats was studied and the report was published by Singapore Medical Association. You can find the detailed report at http://diatea.com/files/testresults1.pdf . I hope this report will get you a great answer if you haven't read it already

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