Why kasumi-1 cells transfected with a plasmid and selected in drug for 21 days died after there multiplycation in a month?

AML cells in general don't transfect very well & typically die shortly after. My guess is that you cultured ghost-cells for 21 days.

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In Microbiology

What are the factors that can affect the transfection efficiency and how?

seeding density of the cell quality of transfecting dna ratio of dna to transfectant reagent

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What practical use can the transfection process be used for?

The transfection process can be used practically in procedures where foreign DNA needs to be introduced into bacteria or mammalian cells. It can be used in investigating gene … ( Full Answer )

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Why kasumi-1 cells transfected with a plasmid and selected in drug for 21 days died after there multiplycation in a month?

What are the factors that can affect the transfection efficiency and how?

What practical use can the transfection process be used for?