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Account for the development of alkalinity in cultures capable of using citrate as their sole carbon source?
Wiki User
∙ 14y ago
Citrate is acted on by the enzyme citrase, which produces oxaloacetic acid and acetate. These products are then enzymatically converted to pyruvic acid and carbon dioxide. During this reaction the meduim becomes alkaline (the carbon dioxide that is generated combines with sodium and water to form sodium carbonate, and alkaline product.
Wiki User
∙ 14y ago
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