The central vacuole of plant cells are really hard to notice, because it makes up most of the cell. However, under a light microscope, it's possible to barely see the space between the cell wall and the central vacuole.
They can only be seen using an electron microscope. A transmission electron microscope can reach magnifications of up to 500,000 times that of a human eye and a thousand times the magnification of a compound microscope. The transmission electron microscope does not use a standard lens to complete its magnifications; instead, it uses a monochromatic beam of electrons passed through a magnetic field, which acts as a lens,
The magnification of a 4 x 12d lens is 48 times
950
The total magnification is equal to the magnification of the eyepiece multiplied by the magnification of the objective lens. So in this case the objective lens would need to be 100X.
The general formula for Total maginifcation Tm is: Tm= Me x Mob where Me is the magnification produced by the eyepiece and Mob is the magnification produced by the objective.
Calculating total magnification is pretty simple. Take the magnification of the eyepiece and multiply by the magnification of the objective. So in this case 10x x 4x = 40x.
At 400X total magnification, we were only able to view one cell at a time, due to the fact that the cells were separated from each other. The organelles that were visible in this type of cell were the nucleus, the cytoplasm and the cell membrane.
Cell wall, nucleus and chloroplasts can be seen with a compound light microscope under a total magnification of 400 X. The chloroplasts are self pigmented hence visible. Cell wall and nucleus being very dense are also visible without staining.
the eyepiece lens magnification X the magnification of the objective lens.
The magnification of a 4 x 12d lens is 48 times
Multiply the magnification of the eyepiece (usually 10x) and the magnification of the objective you are using, Example: eyepiece = 10 x objective lense = 40x 10 x 40 = 400 magnification of 400x.
285 X maximum "usable" magnification
950
The total magnification would be 200x, since the total magnification is the magnification of the objective lens X the magnification of the eyepiece.
To determime total magnification of a drawing you devide the dimensions of the drawing by the dimensions of the real object or specimen drawn Mathematically Magnification =Dimensions of drawing/dimensions of specimen
To calculate magnification , multiply mag.Power of both lenses 15 x 30 = 350
magnification of the eyepiece X magnification of the lens (depends on which one you choose)
magnification.