No. The is the big drawback to SEM and TEM microscopy, the subjects must be fixed to the slide, fractured or some other preparation method and this means they must be dead.
When you need to observe a live specimen
A tunneling electron microscope cannot image live specimens.
Magnification and resolution is in light microscope is about 500x and 0.2micro metres respectively than human eye.In e.microscope magnification is about 200,000x and resolution is 0.0005micro metres. You cannot observe live specimen through electron microscope. Lenses in light microscope are glass while e.microscope use magnetic lenses. E.microscope is relatively very larger than light microscope. You need special knowledge to handle electron microscope. You can observe through naked eye in e.microscope. A beam of electrons is sent through specimen in e.microscope.In light microscope a beam of light is sent. Electron microscope is very complex relative to light microscope.
An electron microscope can reach a far greater magnifiction than a conventional microscope. It is obvious that the largest advantage of any type of microscope is to observe an organism that can not be seen by the unaided eye. Light microscopes are easy to use, can magnify up to 2000 times, enable magnification of live cells and their movement, and are relatively cheap compared to electron microscopes. The only disadvantage is that it can only magnify up to 1000 times, after that the image is blurry, whereas an electron microscope can magnify up to 100,000 times! However, electron microscopes are extremely expensive, difficult to use, and cannot observe live specimens.
A light microscope uses visible light to magnify the specimen, allowing for observation of living cells and tissues. In contrast, an electron microscope uses a beam of electrons to achieve higher resolution and magnification, enabling visualization of smaller structures such as viruses and molecules.
There are at least two types of microscope that can give 3D images. Confocal microscopes that use lasers to illuminate the object and scanning electron microcopes (SEM) that use an electron beam. A SEM can give better magnification than confocal but confocal can image live moving subjects. In SEM the object of intrest must be coated with gold so only dead things can be imaged.
A scanning electron microscope (SEM) provides high-resolution, three-dimensional images of surface topography, allowing detailed examination of sample morphology. A transmission electron microscope (TEM) offers even higher resolution, enabling the visualization of internal structures at the atomic level. In contrast, a light microscope is more accessible and easier to use, making it suitable for observing live cells and larger specimens with lower magnification. Each type of microscope serves specific research needs, balancing resolution, sample preparation complexity, and usability.
Live specimens cannot be used in a transmission electron microscope (TEM) as the high vacuum environment and electron beam would quickly kill any living cells. Instead, biological specimens must be fixed, dehydrated, and stained prior to imaging in a TEM.
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they are relatively smaller, electron ones are biggerthey are easy to usethey allow you to see coloured images, electron ones don'tyou can observe live specimen, specimen has to be dead with electron microscopesi was trying to view the answer in relation to transmmission
You can observe cells using a light microscope, fluorescence microscope, or electron microscope. Each of these tools offers different levels of resolution and the ability to observe different features of cells. Additionally, techniques like immunofluorescence or live cell imaging can provide more specific information about cell structures and functions.
Electron microscope would be used if higher magnification and resolution are required to visualize objects at the nanometer scale. It is suitable for studying the ultrastructure of cells, tissues, and materials with high detail and clarity.