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What is the primary use of deep?

Agar deeps are used to see whether an organism requires oxygen to grow. If there is spreading growth only at the bottom of the tube, the organism is an obligate anaerobe (meaning it cannot tolerate oxygen). If there is growth only at the surface of the agar, the organism is an obligate aerobe (it cannot grow without oxygen). And if there is growth all along the point of innoculation, the organism is a facultative anaerobe and can survive either way.


What is the different between obligate anaerobe and facultative anaerobe?

Facultative anaerobes does not need o2 to grow but can also grow with o2. Obligate anaerobe cannot grow at all in the presence of o2.


Does Haemophilus influenzae grow on blood agar?

Yes, Haemophilus influenzae can grow on blood agar.


What organisms don't grow on broad spectrum nutrient agar?

In my experiments of growing cultures on agar plates, I found Nutrient Agar's usually grow mostly molds and fungi. It's bacteriological growth is wide, yes, but I found that even after a few uses it started growing specifics (mostly gram negative coccus and bacillus).


Can obligate anaerobes grow on a slant that is incubated anaerobically?

Yes, obligate anaerobes can grow on a slant that is incubated anaerobically by using special anaerobic incubation methods such as gas-packs or anaerobic jars to create an oxygen-free environment. These methods allow obligate anaerobes to grow on the slant without being exposed to oxygen.


Will Alcaligenes faecalis grow on MacConkey agar?

No, Alcaligenes faecalis will not grow on MacConkey agar. MacConkey agar is selective for gram-negative bacteria that ferment lactose. Alcaligenes faecalis is a gram-negative bacteria that does not ferment lactose, so it will not grow on MacConkey agar.


What agar does Corynebacterium xerosis grow on?

Corynebacterium xerosis typically grows on nutrient agar.


If an organism can grow on both a nutrient agar and a MacConkey Agar on which would you expect it to grow better?

You would expect the organism to grow better on nutrient agar because it is a general-purpose medium that supports the growth of a wide range of organisms. MacConkey agar, on the other hand, contains inhibitors that selectively inhibit the growth of Gram-negative bacteria, so the organism may not grow as well on this medium.


What do scientists grow bacteria on?

Scientists often grow bacteria on agar plates because agar provides a solid surface for bacteria to thrive on. Agar is composed of nutrients that bacteria need to grow, making it an ideal medium for cultivating and studying bacteria in a controlled environment.


Which bacteria grow in oxygen rich environments?

Obligate Aerobes


Why will obligate anaerobes grow in thioglycollate?

Obligate anaerobes do not need or use oxygen as a nutrient. In fact it is a toxic substance to them which either kills or inhibits their growth. Thioglycollate allows obligate anaerobes to grow because it consists of sodium thioglycollate, thioglycollic acid and L-crystine which reduce oxygen to water. This creates an anaerobic environment allowing obligate anaerobes to grow.


What does it mean to inoculate an agar plate?

Inoculating an agar plate refers to transferring microorganisms onto the surface of the agar using a sterile inoculating loop. This allows the microorganisms to grow and form visible colonies that can be studied or identified.