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Two kinds of creatures are said to be in different species if they cannot successfully interbreed **and** produce offspring that can successfully breed. Many kinds of animals can successfully interbreed. For instance, donkeys and horses can interbreed. The resulting mules cannot reliably breed.

It is unlikely to observe cross-breeding in nature. Tarantula breeders occasionally report that hybrids have been produced.

To interbreed successfully, two species of Spiders would need to be close enough that the female would not regard the male as prey. Differences in appearance, in breeding times, in recognition signals, etc., can all support the differentiation of species. In places where there is no great shortage of either males or females of one species, it is probably pretty unlikely that interbreeding would occur. On the other hand, there is no way to prove that hybrids are never produced.

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