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Lactophenol blue is an important substance used for staining fungi to prepare slides for microscopic observation. It also contains lactic acid to preserve the fungal structures while the blue tint makes them easier to discern.
Lactophenol Cotton Blue is recommended for mounting and staining yeast and molds. It is formulated with lactophenol, which serves as a mounting fluid, and cotton blue. Organisms suspended in the stain are killed due to the presence of phenol. The high concentration of the phenol deactivates lytic cellular enzymes thus the cells do not lyse. Cotton blue is an acid dye that stains the chitin present in the cell walls of fungi.