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Q: Define resolution separating of specimen at which they become two separate entities?
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What is the shortest distance between two points on a specimen at which they are resolved as two separate entities?

resolution


What is meant by limit of resolution?

It is defined as the capacity of an optical system to resolve point objects as separate objects. Larger the aperture of optical system greater will be its limits of resolution.


What part of a microscope regulates resolution and definition of the specimen?

The Iris Diaphragm.


Fuzzy images viewed by the microscope are due to poor?

Fuzzy images viewed with a microscope may be due to poor resolution. In microscopy, the resolution is defined as the smallest distance between two points on a specimen that can still be defined as two separate objects.


As the magnification increases what decreases in a microscope?

The visibility of the specimen decreases as the power of magnification increases on a microscope. The specimen area will shrink as the magnification is increased.


What influences resolution in regards to the compound binocular light microscope?

Resolution of a microscope is tied to the numerical aperture of the objective lens and the condenser but is influenced by other factors, such as alignment, type of specimen, wavelength of light, and contrast enhancing techniques. Read more: Define Resolution in Microscopes | eHow.com http://www.ehow.com/facts_5753341_define-resolution-microscopes.html#ixzz1kYyrj6D9


When you move to a higher magnification do you see more or less of the specimen?

With higher magnification you can observe bigger.To see clearly resolution also should be high.


Oil immersion is used as a medium between the specimen and the oil immersion objective lens why?

Oil immersion is a technique used to increase the resolution of a microscope.


What are the disadvantages of fluorescence microscope?

Cannot provide spatial resolution below the diffraction limit of specific specimen features http://www.microscopyu.com/articles/fluorescence/fluorescenceintro.html


How is clotted blood obtained?

Clotted blood is normally obtained from a specimen of venous blood which has been allowed to clot by standing for 30 minutes. The resulting specimen is usually centrifuged to separate the clotted red blood cells from the serum.


What is the purpose of staining a specimen when using the microscope?

Staining a specimen allows you to better see what exactly is under the microscope. It helps to separate the specimen and the slide underneath or on top. Another way to think of this is like is it easier to see white sprinkles on white frosting, or blue sprinkles? Blue, of course! It's just easier to see.


What happens to the image when you increase magnification?

In a light microscope when magnifiaction increases resolution decreases and the object will apear blurred. It can be removed by putting immersion oil on slides or object which increase the refractive index and cause to increase the numerical aperture which ultimately cause the better resolution as resolution power depends on numerical aperture of lens. The immersion oil used can be cedar oil.