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It is defined as the capacity of an optical system to resolve point objects as separate objects. Larger the aperture of optical system greater will be its limits of resolution.
The Iris Diaphragm.
The visibility of the specimen decreases as the power of magnification increases on a microscope. The specimen area will shrink as the magnification is increased.
With higher magnification you can observe bigger.To see clearly resolution also should be high.
low power means we observed specimen at 40x and for more clear result we go to high resolution wich is 100x with the use of oil emmersion
resolution
It is defined as the capacity of an optical system to resolve point objects as separate objects. Larger the aperture of optical system greater will be its limits of resolution.
The Iris Diaphragm.
Fuzzy images viewed with a microscope may be due to poor resolution. In microscopy, the resolution is defined as the smallest distance between two points on a specimen that can still be defined as two separate objects.
The visibility of the specimen decreases as the power of magnification increases on a microscope. The specimen area will shrink as the magnification is increased.
Resolution of a microscope is tied to the numerical aperture of the objective lens and the condenser but is influenced by other factors, such as alignment, type of specimen, wavelength of light, and contrast enhancing techniques. Read more: Define Resolution in Microscopes | eHow.com http://www.ehow.com/facts_5753341_define-resolution-microscopes.html#ixzz1kYyrj6D9
With higher magnification you can observe bigger.To see clearly resolution also should be high.
Oil immersion is a technique used to increase the resolution of a microscope.
Cannot provide spatial resolution below the diffraction limit of specific specimen features http://www.microscopyu.com/articles/fluorescence/fluorescenceintro.html
Clotted blood is normally obtained from a specimen of venous blood which has been allowed to clot by standing for 30 minutes. The resulting specimen is usually centrifuged to separate the clotted red blood cells from the serum.
Staining a specimen allows you to better see what exactly is under the microscope. It helps to separate the specimen and the slide underneath or on top. Another way to think of this is like is it easier to see white sprinkles on white frosting, or blue sprinkles? Blue, of course! It's just easier to see.
In a light microscope when magnifiaction increases resolution decreases and the object will apear blurred. It can be removed by putting immersion oil on slides or object which increase the refractive index and cause to increase the numerical aperture which ultimately cause the better resolution as resolution power depends on numerical aperture of lens. The immersion oil used can be cedar oil.