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A gram-negative cell will lose its outer membrane and the peptidoglycan layer is left exposed.
or it is best to use younger cells ( 12-24hr) because older gram positive bacteria are subject to break down of the cell wall by enzymes that are produced with age which may result ingram variable staining.
Wiki User
∙ 13y ago
Wiki User
∙ 11y agoGram variability of bacteria is a function of the cell wall composition. For gram positive bacteria (they stain in violet!) the cell wall is composed of peptidoglycan and they do not have a lipopolysaccharide layer above this peptidoglycan layer. Gram negative bacteria (stain pink!) have a lipopolysaccharide layer above the peotidoglycan layer which do stain violet with crystal violet, but are washed off by the ethanol treatment. The peptidoglycan layer beneath remains untouched by the crystal violet stain and is now free to take up the pink color of safranin.
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What does gram negative on a serratia marcescens gram stain mean?
Bacteria are gram positive or gram negative. Serratia happens to be a gram negative bacteria. They appear pink on a gram stain. Gram positive bacteria stain to a purple color on a gram stain. We can classify and ID bacteria using their gram stain and shape. Some antibiotics only work on gram negative bacteria and some only work on gram positive bacteria. It helps a doctor know which antibiotic to use.
What is the different reaction of Gram-negative and Gram-positive bacteria to Gram's stain testing?
Gram-positive bacteria which take up the stain turn purple, while Gram-negative bacteria which do not take up the stain turn red.
Why would you want to know if a gram stain is positive or negative?
Gram negative bacteria (pink gram stain) contain no outer cell membrane, while gram positive bacteria (purple gram stain) do contain an outer cell membrane. Gram negative and positive bacteria can respond differently to antibiotics. Many only work on only one of the two bacteria types. A gram stain is also the first step in identifying a bacteria, dividing bacteria into two large and distinct groups.
Why would a gram stain be ordered during surgery?
to determine a type of bacteria. This question is very vague. I will assume they are doing surgery on something that is infected and to help identify the the bacteria causing the infection, gram stain would be ordered.
What color is Gram- stain?
Gram- negative bacteria turns red to pink after the gram stain is performed.
What does gram negative on a serratia marcescens gram stain mean?
Bacteria are gram positive or gram negative. Serratia happens to be a gram negative bacteria. They appear pink on a gram stain. Gram positive bacteria stain to a purple color on a gram stain. We can classify and ID bacteria using their gram stain and shape. Some antibiotics only work on gram negative bacteria and some only work on gram positive bacteria. It helps a doctor know which antibiotic to use.
What is the gram stain of H5N1?
The Gram stain is used for bacteria and not for viruses.
What is the different reaction of Gram-negative and Gram-positive bacteria to Gram's stain testing?
Gram-positive bacteria which take up the stain turn purple, while Gram-negative bacteria which do not take up the stain turn red.
Why would you want to know if a gram stain is positive or negative?
Gram negative bacteria (pink gram stain) contain no outer cell membrane, while gram positive bacteria (purple gram stain) do contain an outer cell membrane. Gram negative and positive bacteria can respond differently to antibiotics. Many only work on only one of the two bacteria types. A gram stain is also the first step in identifying a bacteria, dividing bacteria into two large and distinct groups.
When treated with Gram stain Gram-positive bacteria appear?
Gram positive bacteria retain the violet stain, and appear purplish under a microscope.
Does the gram stain procedure work for all bacteria?
Yes. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which do stain purple. In structural terms, the ability of a cell to become stained during the gram stain procedure is due to the chemical makeup of the cell wall.
Why would a gram stain be ordered during surgery?
to determine a type of bacteria. This question is very vague. I will assume they are doing surgery on something that is infected and to help identify the the bacteria causing the infection, gram stain would be ordered.
What color is Gram- stain?
Gram- negative bacteria turns red to pink after the gram stain is performed.
What is the Gram stain for measles?
Bacteria stain either gram-positive or gram-negative based on the presence or absence of a cell wall. Viruses do not pick up a gram stain.
Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
Gram stain of the bacteria vibrio cholerae?
It's gram negative
What is the advantage of the gram stain over the simple stain?
gram staining differentiates between gram negative and gram positive bacteria by showing different colors. it shows blue or purple like color for gram positive bacteria and red color for gram negative bacteria. where as simple stain gives the same color to all the types of bacteria. hence it is difficult to differentiate between them.
