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the chamber has a positive end and a negative end...the DNA moves through the gel toward the positive end (because DNA is negative). The smaller fragments move faster, therefore going further, and the larger pieces stay closer to the wells.
what is DNA gel
Wiki User
∙ 12y ago
Wiki User
∙ 15y agoThis depends on how u made your gel, volts it ran, how long, DNA size AND it can also depend on if the DNA is coiled or linear
Imagine the gel like an obstacle course, what makes it farthest to the end are the smallest pieces because they can get there easier.
Also be careful of having more then one piece of DNA being shown. This can happen if your restriction enzyme happens to cut the same size pieces and will stain darker
If your question is about staining, Ethidium Bromide (usually) inserts itself in the DNA to glow so it can be observed.
Wiki User
∙ 14y agoGel electrophoresis is based on separating DNA fragments under the influence of an electric field. DNA is negatively charged and therefore moves toward the positive electrode when placed in an electric field.
This process is carried out in a gel made of agarose (a complex polyscaaharide). DNA is added at one terminal end of the gel into small wells etched out in the gel. The end of the gel where DNA is added is called the origin. The opposite end is called the terminal end.
When the gel is placed in an appropriate buffer (TBE is most cases) and connected to electrodes, the DNA fragments move from the origin to the terminal end. After the process is complete. the smaller fragments (that move faster through the gel) are found closer to the terminal end whereas the larger fragments (that move slower) are found closer to the origin. This is how DNA fragments are separated based on size.
Wiki User
∙ 11y agoNegatively charged DNA travels to the anode which is positive in charge.
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Explain how an agarose gel can separate DNA fragments of different lengths.?
The separation of DNA fragments is based on size. When a DNA sample is run in a gel (electrophoresis), the lighter fragments migrate faster than the heavier (longer) fragments under the influence of an electric current. At the and of the process, the shorter fragments are found at the terminal end of the gel and the longer fragments closer to the origin
What enzyme binds fragments of DNA on the lagging strand?
The enzyme that is used to bind DNA fragments together is DNA ligase. Using DNA ligase to join DNA fragments is the last step in the production of a recombinant DNA plasmid.
How do restriction sites and a restriction map relate?
Restriction maps show the lengths of DNA fragments between restriction sites in a strand of DNA.
If you have a restriction enzyme that cuts a piece of DNA at two recognition sites how many DNA fragments would you see on a gel?
Three.To see why, cut a piece of string in two places! Of course, strictly you would not be able to see only three fragments. You would amplify the DNA before carrying out electrophoresis. That way, you would get perhaps 200 million copies of each fragment, and they would show up. Also, you would only be able to distinguish the fragments if they were different lengths. Electrophoresis separates pieces of DNA by length.
Analysis of fragments of DNA as a form of identification?
DNA Fingerprinting
What does the charge of DNA have to do with DNA fingerprinting?
DNA is of a negative charge. So when gel electrophoresis is used on it the DNA fragments are attracted to the positive end of the electrophoresis. The fragments of different lengths travel down the gel towards this end. The longer length fragments travel less and so are farther from the positive end. By looking at these DNA fragments, which are created by cutting DNA with restriction enzymes one can compare and contrast DNA. Thus DNA fingerprinting can take place based on the different restriction sites in DNA (cut by the enzymes) forming different length segments of DNA.
Explain how an agarose gel can separate DNA fragments of different lengths.?
The separation of DNA fragments is based on size. When a DNA sample is run in a gel (electrophoresis), the lighter fragments migrate faster than the heavier (longer) fragments under the influence of an electric current. At the and of the process, the shorter fragments are found at the terminal end of the gel and the longer fragments closer to the origin
How are restriction maps used?
They are used to show the lengths of DNA fragments between restriction sites in a strand of DNA.
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
How do you get DNA fragments in Bakugan dimensions?
You get DNA fragments by entering Bakugan codes.
Does a restriction enzyme generate the same size fragments in genomic DNA of different species?
No. A restriction enzyme cuts DNA when it finds a specific sequence. Different animals will have these sequences occur at different intervals so the length of the fragments won't be the same.
What do you do with DNA fragments in bakugan dimensions?
When You collect 20 DNA fragments you get a free bakugan
What are DNA fragments that have attached RNA fragments called?
Okazaki fragments.
A collection of host cells that house different cloned fragments of DNA?
gene library
Does Fragments of DNA from two different organisms have been joined to form a genome?
yes
What enzyme binds fragments of DNA on the lagging strand?
The enzyme that is used to bind DNA fragments together is DNA ligase. Using DNA ligase to join DNA fragments is the last step in the production of a recombinant DNA plasmid.
How do you use the DNA fragments on bakugan dimensions?
You have to collect 20 DNA fragments to get a free bakugan
