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Benedict's test is based on Benedict's reagent, a liquid that changes color based on the sugar content of the material put in. Ketohexose acts as a reducing sugar because benedict's reagent is comprised of sodium citrate, sodium carbonate, and cupric sulfate. The cupric sulfate reacts with the ketohexose, reducing it and causing it to precipitate as cuprous oxide, which is what changes the color of the reagent and determines the sugar content.
Wiki User
∙ 9y ago
Wiki User
∙ 11y agoBenedict's 2 Cu2+ and 5 OH- indicates the presence of a of a oxidizing sugar. The copper gets reduced and sugar gets oxidized. If glucose was oxidized with Benedict's it would form gluconate due to the loss of hydrogen. The solution goes from blue to red. Red indicates the presence of the anion showing that the sugar originally present was able to be oxidized.
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What chemical is used to test for reducing sugar?
Benedict's solution is used to test for reducing sugar.
What compound can be oxidized by both Benedicts solution and Tollens reagent?
All the compounds having free aldehyde group as reducing sugars.
What sugar might be present in a sweet tasting food that tests negative with the Benedicts reagent?
Sucrose. Sucrose is a disaccharide therefore does not have free electrons in the sugar to react with the Benedict reagent. Glucose has free electrons therefore shows positive with the Benedict reagent.
What is the difference between Benedict's Reagent and Biuret Reagent?
Benedicts reagent is used to check reducing sugars , it need hat to work and gives red color when positive ,. While biuret reagent is for proteins .there is no need to use heat and gives purple , violet color when positive ..
Would glucose solution produce a positive Benedicts test?
No, Benedicts reagent will show positive results if the carbohydrate is a reducing sugar. You will know if it is positive if the sample will turn from blue to green then to orange when you are cooling the solution, which is the last step when you are performing the benedicts test for carbohydrates.
Would raffinose react with benedicts reagent?
Benedicts reagent tests for reducing sugars, so the question is, is raffinose a reducing sugar. Raffinose is a trisaccharide made up of glucose, fructose and galactose. It is not a reducing sugar because all of its anomeric carbons are bonded, so it will not react with benedicts reagent.
What chemical is used to test for reducing sugar?
Benedict's solution is used to test for reducing sugar.
Will hydrolysed dextrine give positive response to Benedict test?
Yes, it should. Benedicts test will be positive for reducing sugars, and since glucose is such a sugar, and would be a product of dextrin hydrolysis, you should get a positive result with Benedicts reagent.
If benedicts reagent turns red what substance is present?
the sugar glucose
Which carbohydrates give a positive reaction with benedicts reagent?
all reducing sugars - monosaccharides like glucose and a few non-reducing disaccharides like maltose
If corn oil is tested with Benedicts's solution and Biuret reagent and the final color is blue what do you know about corn oil?
Benedict's solution tests for aldehyde which is present in reducing sugars. If the solution remained blue than no reducing sugar's are present in corn oil.
What compound can be oxidized by both Benedicts solution and Tollens reagent?
All the compounds having free aldehyde group as reducing sugars.
What does Benedicts reagent do?
It tests for glucose.
What sugar might be present in a sweet tasting food that tests negative with the Benedicts reagent?
Sucrose. Sucrose is a disaccharide therefore does not have free electrons in the sugar to react with the Benedict reagent. Glucose has free electrons therefore shows positive with the Benedict reagent.
What is the difference between Benedict's Reagent and Biuret Reagent?
Benedicts reagent is used to check reducing sugars , it need hat to work and gives red color when positive ,. While biuret reagent is for proteins .there is no need to use heat and gives purple , violet color when positive ..
Would glucose solution produce a positive Benedicts test?
No, Benedicts reagent will show positive results if the carbohydrate is a reducing sugar. You will know if it is positive if the sample will turn from blue to green then to orange when you are cooling the solution, which is the last step when you are performing the benedicts test for carbohydrates.
A reducing sugar gives a precipitate of silver metal with Benedict's reagent.?
This is probable an error.
