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The difference between the coding strand and the template strand is the coding strand is the strand which contains the coding genes, i.e. the one in which the RNA polymerase reads and transcribes into mRNA. It must have the promoter sequence in the correct orientation for transcription, as follows:

5`-TATAATGCGCGCGCGCGCGCGCGC-3`
3`-ATATTACGCGCGCGCGCGCGCGCG-5`

In this sequence, the top strand is the coding strand, because it contains the promoter (TATAAT) in the correct orientation.
However, when transcribed, the mRNA will be as follows:

5`-GCGCGCGCGCGCGCGCGCGC-3`

This is because the polymerase transcribes from the template strand, on the opposide side to the coding strand, to make it in the same orientation as the coding strand.

I hope I have explained it enough for people to understand, however if I haven't please read this article I found which explains it thoroughly:

http://www.bio.net/bionet/mm/bioforum/1994-May/008821.html

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15y ago

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What is the coding DNA and mrna strand for the template strand 3' a-g-g-t-t-c-a-t 5'?

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