coomassie brilliant blue dye is a linear dye whereas the silver stain dye is a nonlinear one. Besides that coomassie dye has a lower sensitivity and is used for detection of highly abundant proteins. Silver stain relies on adsorption rather than absorption and is more suitable for detection of low abundant proteins but with a higher sensitivity.
A stain is temporary whereas dyes are permanent and can be removedonly after cell wall destruction.
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Well a no name brand doesn't have a name but a brand name does
in Zn we use 3% decolorizer, while in modified 1% h2so4 is used, modified is used for paracite like Cryptosporidium, cyclosporidium and bacterias like nocaddia and actinomyces. also in modified heating isn't done.
Silver ions (from silver nitrate in the stain reagent) interact and bind with certain protein functional groups. Strongest interactions occur with carboxylic acid groups ( Asp and Glu), imidazole (His), sulfhydryls (Cys), and amines (Lys)
The difference between Wright Stain and Giemsa Stain is the intensity of the stain. The Giemsa Stain provides a better stain intensity than the Giemsa stain.
In coomassie staining, a dye binds to all proteins, visualizing all of them. In this way, it's nonspecific. The dye diffuses into the the original gel. In a western, an antibody binds specifically to one or more proteins, making it specific for proteins to which the antibody can bind. In order for the antibody to access the proteins from the original gel, they are moved (blotted) to the surface of a membrane (usually nitrocellulose).
A stain is temporary whereas dyes are permanent and can be removedonly after cell wall destruction.
A stain is used to color the wood while a varnish is a topcoat used to protect the wood.
You can an electrophoresis gel and then stain the gel using a solution such as coomassie blue to make the bands visible. Alternatively, you can stain a cell containing DNA by using acridine orange. It is necessary to observe these under an electron light microscope.
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The difference between normal dirt and a stain is in their difficult to wash off. Normal dirt comes off easily but a stain is a colored patch which penetrative and thus making it hard to remove.
Protists are often stained using a silver stain, not a Gram stain.
Wright's Stain is a mixture of methylene blue and eosin in methanol. Gram's stain is crystal violet, iodine washed with acetone and proofed with a safranin dye to look for gram negative organism.
its the difference in cell wall of both the type of bacteria
a dye is used on inanimate objects & stain is used on animate objects .
The difference between Dieing and Dying is that Dieing means to stain with color. Cut, form shape with a die as in metal. Dying means passing away as in death.