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This question has to be answered depending upon the meaning of a research project that involves proteins.
The estimation of proteins can be done to know the protein fraction of a sample collected from the field, either if the protein content will be isolated to be studied, or to remove protein fraction from the sample (sometimes the investigator does not want that proteolytic enzymes chew a target molecules or cellular structures).
Another application is when an enzymatic reaction is going to be performed to digest a particular protein in an aliquot, the protein content is crucial for stoichometric purposes.
On the other hand, if the research imply the work with nucleic acids, is very important "to inactivate" the nucleolytic enzymes present in cytoplasmic fluids from lysed cells with specific inactivators, chemical or biochemical, added in correct levels according to protein contents.
What else can I help you with?
Is lowry's protein estimation an invasive method?
No, Lowry's protein estimation method is not invasive. It is a biochemical method used to quantify the total protein concentration in a sample based on the reaction of proteins with specific reagents. It does not involve physical penetration or manipulation of the sample.
Why protein estimation is done at 660 nanometer in folin lowry method?
Protein estimation in the Folin-Lowry method is done at 660 nanometers because this wavelength corresponds to the highest absorption peak of the complex formed between proteins and the Folin-Lowry reagent. This wavelength is ideal for accurately measuring the concentration of proteins present in a sample based on the colorimetric reaction that occurs.
What is chemical estimation ATP?
Chemical estimation of ATP refers to methods used to quantify the amount of ATP (adenosine triphosphate) present in a sample. This can be done using various assays, such as bioluminescence assays, which detect the light emitted when ATP reacts with luciferase enzyme, or colorimetric assays, which measure the production of colored compounds when ATP reacts with specific enzymes. These methods are important for understanding cellular energy metabolism and are commonly used in research and diagnostics.
What factors influence the protein yield in a bioprocess?
Factors that influence protein yield in a bioprocess include the type of organism used, the growth conditions, the fermentation process, and the purification methods employed.
What are the key methods used to determine the protein crystal structure?
The key methods used to determine protein crystal structure include X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and cryo-electron microscopy (cryo-EM). X-ray crystallography involves analyzing the diffraction pattern of X-rays passing through a protein crystal. NMR spectroscopy detects the interactions between atoms in a protein to determine its structure in solution. Cryo-EM uses electron beams to visualize protein structures at near-atomic resolution.
What are the methods in estimation?
There are a few methods in estimation. Like framework, unknown parameters, empirical dist and substitution principle most of these methods can be used using substitution principles.
What is an invasive method of protein estimation?
gand mara
What are the methods of estimation of reducing sugar?
by comparing the colours or the amount of precipitate
What is protein estimation?
here the amount of protein in a sample can be determined, using different protocols, wherein the reagent mixture( ex: FC reagent) which when added to the sample containing protein reacts with the specific amino acids giving colour, thus the amount of protein in a sample can be estimated and the data used for further protein studies.
Methods for the estimation of Nacl?
For example concentration determination with a salinometer.
Why we are used sodium bicarbonate for the estimation of protein?
Sodium bicarbonate is not necessarily used in the estimation of protein. There are very many protein assays on the market, and the vast majority of them do NOT use NaHCO3. Look up Lowry, Biuret, Bradford, etc.
What are the alternative methods to western blot for protein detection and analysis?
Alternative methods to western blot for protein detection and analysis include enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), mass spectrometry, and protein microarrays. These methods offer different advantages such as higher sensitivity, multiplexing capabilities, and the ability to analyze protein interactions.
What are the different types of protein purification methods available?
There are several types of protein purification methods available, including chromatography, electrophoresis, ultrafiltration, and precipitation. Each method has its own advantages and is used based on the specific characteristics of the protein being purified.
Is lowry's protein estimation an invasive method?
No, Lowry's protein estimation method is not invasive. It is a biochemical method used to quantify the total protein concentration in a sample based on the reaction of proteins with specific reagents. It does not involve physical penetration or manipulation of the sample.
What is determining the value or amount of something by mathematical or logical methods?
There is no single word. Mathematical methods, alone, could involve analytical methods, statistical methods of estimation or numerical methods for approximation.
Latest methods of screening methods for Antiepileptic drugs?
High Performance Liquid Chromatography can be used for the estimation ofmost of the antiepilleptic drugs
What has the author O Richter written?
O. Richter has written: 'Parameter estimation in ecology' -- subject(s): Ecology, Mathematical models, Parameter estimation, Statistical methods
