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here the amount of protein in a sample can be determined, using different protocols, wherein the reagent mixture( ex: FC reagent) which when added to the sample containing protein reacts with the specific amino acids giving colour, thus the amount of protein in a sample can be estimated and the data used for further protein studies.
What else can I help you with?
What method are used for the estimation of molecular weight of protein?
ofcoz the protein migrate so long as it is charged ,once it become neutral it will stop migrating
What are Three factors that affect range estimation?
Three factors that affect range estimation are visibility conditions (such as fog or smoke), the size and shape of the target object, and the experience/training level of the individual making the estimation.
What are the contribution pf chemistry to meteorology?
It allows for the estimation of the greenhouse effect and the formation of clouds.
What types of protein are made through protein synthesis?
protein
What is the characteristic of protein?
analysing the function of a particular protein is protein characterization.
What is an invasive method of protein estimation?
gand mara
Why we are used sodium bicarbonate for the estimation of protein?
Sodium bicarbonate is not necessarily used in the estimation of protein. There are very many protein assays on the market, and the vast majority of them do NOT use NaHCO3. Look up Lowry, Biuret, Bradford, etc.
Is lowry's protein estimation an invasive method?
No, Lowry's protein estimation method is not invasive. It is a biochemical method used to quantify the total protein concentration in a sample based on the reaction of proteins with specific reagents. It does not involve physical penetration or manipulation of the sample.
What percent of snake venom is protein?
Average protein estimation in snake venom is from 49.8 to 96.4% . the age of the snake may affect the percentage of protein content found in the venom
Role of F C reagent in protein estimation?
phosphomolybdic & phosphotungstic components in FC reagents gets reduced by certain amino acids present in the protein like tyrosine , tryptophan thereby resulting in the production of a a blue colored compound . Estimation of this compound in turn provides the amount of protein present in the sample.
What method are used for the estimation of molecular weight of protein?
ofcoz the protein migrate so long as it is charged ,once it become neutral it will stop migrating
How long the blue colour is stable in protein estimation by lowry's method?
60 minutes 60 minutes
Why BSA is used as standard for protein estimation?
The Bradford assay (BSA) is commonly used for protein estimation because it is quick, sensitive, and compatible with a wide range of proteins. BSA is a reliable method for measuring protein concentration due to its colorimetric detection of the dye-protein complex, making it a popular choice in biochemical research and diagnostics.
Why is protein estimation needed in Diabetes?
Its basically depends on weight of the body balancing with nitrogen gas of the body whcih balance sugar level.
What is lambda max for colorless proteins?
if the protein is colorless then it is quite tough to calculate the lambda max or absorption maxima directly (still you can check it by absorbtion maxima calculation/experiment). Otherwise, for its estimation first you have to treat it with any reagent (like folin reagent) then it will form a colored complex and then you can calculate it. For the common estimation of protein, there is a "Lowry Method" where you can calculate it on 750 wavelength.
What is approximate estimation?
If an estimation, or estimate, is a guess, an approximate estimation is a rougher guess.
What is the estimation of 328000?
What is the estimation 328000
