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We can hardly differentiate the four lines drawn within a one-milimeter-length (250 micrometer). Below this line lies the realm which is invisible to human unaided eye: 200-250 micrometer

The resolution of the light microscope cannot be smaller than the half of the wavelength of the visible light, which is 0.4-0.7 micrometer. When we use green light (0.4 micron), we can see the objects which is, at most, about 0.2 micron (200 nanometer). Below this point, light microscope is useless, because we must use a wavelength smaller than 400 micrometer. The waves that associate the electrons has smaller wavelength. Then we can use electrons, but in an electron microscope.

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14y ago
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12y ago

The unaided human eye= 0.2mm whereas the microscope is 0.2 micrometers

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Q: What is the limit of resolution of light microscope and that of the unaided eye?
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Related questions

What is the resolution limit of the electron microscope?

50 picometers (pm)


The high dry objective lens has a numerical aperture of 0.85 what is the limit of resolution on this microscope?

The limit resolution is (520/2.1)= 247.61 or 248


What do you men by resolutionpower?

Resolution power (optics) is the reciprocal of limit of resolution.It is described for optical instruments like microscope,telescope etc. Limit of resolution is the minimum distance between two closely placed objects so that their image can be viewed distinctly by a microscope.


What allows the user of a microscope to limit the amount of light from a steady light source?

Diaphragm


Is there an upper limit to magnification using visible light?

Yes, the wavelength of the light limits the maximum magnification of a microscope. Using visible light, the limit is about 1200 to 1500X.


What is the maximum resolution of light in a light microscope in micrometers?

Very roughly, the limit is determined by the wavelength (colour) of the electromagnetic spectrum (light) it uses and will be about 1/2 this wavelength. For more detail the link below is good.


Can a light microscope magnfiply up to 10000?

No ten thousand is too far, given the wavelength of light, the limiting factor for optical microscopes. Perhaps a 1200 magnification is the practical limit for a simple light microscope.


What are the disadvantages of fluorescence microscope?

Cannot provide spatial resolution below the diffraction limit of specific specimen features http://www.microscopyu.com/articles/fluorescence/fluorescenceintro.html


Why is the mitochondria and chloroplast not visible under a micro-scope?

Microscopes have been crucial for understanding organelles. ... However, most organelles are not clearly visible by light microscopy, and those that can be seen (such as the nucleus, mitochondria and Golgi) can't be studied in detail because their size is close to the limit of resolution of the light microscope.


Can neptune be seen with out a telescope at night?

No. Its brightness is below the limit of sensitivity for the unaided human eye.


What factors determine the limit of resolution of a light microscope?

The limit of resolving power of a microscope is described by the Abbe criterion: d=wl/NA d being the minimal resolvable distance between two spots of the object wl being the wavelength of the light used NA being the numerical aperture of the microscope, which is equal to n*sin(a) with n being the refraction index of the immersion liquid between object and objective a being the aperture angle because sin(a) is always smaller than 1 and n cannot rise above 1.7, the maximal resolving power of a microscope is about d=wl/2 and thus only depends on the wavelength of the light used, which normally will be about 600 nm.


What is the light microscope's magnification?

That means how much larger you see something, compared to seeing it with the naked eye. The limit for USEFUL magnification is about a thousand, in the case of hte light telescope.