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Discontinuous gel in analysis of protein?

Updated: 8/21/2019
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Why discontinuous gel system is more favorable in the analysis of protein

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Q: Discontinuous gel in analysis of protein?
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Why agarose gel electrophoresis is not used for protein analysis?

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What is the function of the gel?

Separating gel allows the separation of protein molecules according to their molecular weight by sieving effect of pores in the gel(percentage). The pH of separating or resolving gel is 8.8, whereas stacking gel (upper gel that squeezes protein as a thin layer) made of pH6.8.


What is protein profiling?

protein profiling using 2d gel electrophorosis


Is the length of hair continuous or discontinuous?

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Is the gender a continuous or discontinuous variation?

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What is blotting and types of blotting?

Blotting is a technique of transferring DNA or RNA or Protein from a gel to the membrane. nitrocellulose, PVDF or nylon membrane are used to attach these molecules permanently on them. Western, southern and norther blotting are the techniques used to transfer protein, DNA and RNA respectively. It is done to detect them with a specific probe or antibody. This can not be detected or easy to handle on the gel, so we do blot for these analysis.


Does ampro styl gel make hair hard?

Yes it does because it contains protein. There are some gel that is soft such as softee and other brands that will not make your hair hard but still contain protein.


Is human skin colour continuous or discontinuous variation?

Natural eye colour is discontinuous. :) !


Is rose flower color continuous or discontinuous variation?

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What is proximate analysis of protein?

proximate analysis of fat can be done by using soxhlet apparatus


What is native gel electrophoresis?

Native Gel or the Native PAGE is the electrophoretic system in which the the proteins are run in their native conformation, that is that they are not denatured. This used when the function of the protein is important, especially enzymes, as the function of a protein is related to its native structure.


What is gel electrophoroses?

The migration of DNA/Protein on the gel (agarose/polyacrylamide) by the influence of electric charge is called gel electrophoresis. It is used to resolve the biomolecules according to their size(mainly) and shape(for proteins)