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resolving power
Melting points are very different.
It is not necessary - it is a convention to distinguish between the end points of a range being included or not in the solution.
yes!
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No, you will not be able to distinguish two points that are 300 nm apart as being separate using standard optical microscopy, due to the diffraction limit of light, which is typically around 200-250 nm. Techniques like electron microscopy, which do not rely on visible light, can resolve much smaller distances and could distinguish points that close together.
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The ability of a lens system to distinguish two points is referred to as its resolution. This is determined by factors such as the wavelength of light used and the numerical aperture of the lens. Higher resolution allows for clearer images with finer details, enabling the lens system to differentiate between closely spaced points. In optical systems, resolution is often quantified using the Rayleigh criterion, which defines the minimum distance at which two point sources can be resolved.
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Whether two points that are 300 nm apart can be distinguished as separate depends on the resolving power of the imaging technique used. For optical microscopy, the diffraction limit typically allows resolution down to about 200 nm, meaning that points 300 nm apart may appear as a single blur. However, techniques like electron microscopy, which have higher resolution capabilities, could distinguish them as separate points. Thus, the ability to distinguish depends on the method employed.
Resolution in a microscope refers to the minimum distance between two points that can still be distinguished as separate entities. It is a measure of the microscope's ability to distinguish fine details in an image. A higher resolution means that the microscope can provide clearer and more detailed images with better clarity and sharpness.
According to Wikipedia (see link), a human eye with excellent acuity can distinguish between lines that are 0.35 mm apart, held at a distance of 1 meter. That is 350 micrometres or 350,000 nm - somewhat larger than the 250 nm in the question!