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Yes. In order for a female dog to become pregant, she must "tie" with the male; that is, the male's penis swells for a short time and cannot be removed from the bitch's vagina. This happens to prevent another male from successfully breeding with the female. However, a bitch is in heat for up to two weeks, and it is possible for another dog to tie with her during that time. This can result in a litter of puppies that look as if they had two different fathers, one a beagle and one a chow, for example. It is also theoretically possible for more than two male dogs to sire one litter.

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