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Land clearing affects Northern Quolls to some extent, as it does all quolls. Land clearing not only removes the quolls' shelter, but it reduces the number of hiding places for the quolls' prey, so the prey moves on to safer areas.

However, there are two more significant threats to the Northern Quoll. The Northern quoll is particularly affected by fire, due largely to the introduction of the weed Gamba Grass. Gamba Grass is highly invasive, out-competing native grasses. It affects the Northern quoll because it is a high biomass pasture grass that tends to promote very hot, intense fires during the late dry season. Gamba Grass fires are eight times hotter and more intense than those produced by native grasses. This poses a risk to the Northern Quoll by either killing them directly in the fires, or removing the availability of shelter and habitat to protect the quoll from larger predators and Birds of Prey.

Another major threat to Northern Quolls is that, as their food source is reduced (largely by land clearing), they ate forced to find alternative food, and one of these is the cane toad. Their population is declining significantly as a result of them eating the Cane Toad, which is poisonous when ingested.

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