the light from the lamp below the table would not get through the sample, meaning it could not be seen or analysed.
In some cases, nothing can be seen through a microscope if the sample is too thick or dense for light to pass through effectively. Additionally, if the sample is not mounted properly or if the microscope is not focused correctly, it can also result in not being able to see anything. Proper sample preparation and microscope settings are important for clear visualization.
A Light stereoscope microscope; this type of microscope is used to see three dimensional views of objects too thick to let light pass through.
A Light stereoscope microscope; this type of microscope is used to see three dimensional views of objects too thick to let light pass through.
If the object is too thick for light to pass through, the light would be blocked and unable to reach the objective lens. As a result, the object would not be visible or would appear very dark and blurry under the microscope. Adjusting the focus or trying a thinner sample would be necessary to obtain a clearer image.
You would use a scanning electron microscope (SEM) to examine thick objects that do not allow light to pass through them. SEM uses electron beams instead of light to create detailed images of the surface of the object.
Cutting a thin slice of the specimen allows light to easily pass through the sample and provides a clearer image under the light microscope. A thin slice also ensures that the entire specimen can be viewed at once, without the need to focus on different depths. This method helps in obtaining detailed and high-quality images of the sample.
The resolution of the microscope, which is determined by factors like the wavelength of light used and the numerical aperture of the lens, sets a limit on how small the details can be resolved. Additionally, the contrast between different structures in the sample can impact visibility, with stains or special techniques sometimes needed to enhance contrast. The sample itself may also present limitations, such as being too thick for light to pass through effectively.
If you look at a thick opaque object through a compound microscope, you would likely see little to no details as the object is blocking the passage of light. Additionally, the object may appear dark or shadowed since light cannot pass through it to form an image on the microscope's lens.
You would use a compound microscope with transmitted illumination or a digital microscope with top lighting. These microscopes can illuminate the object from above, allowing you to observe details even if the object is too thick to let light pass through it.
With a normal light microscope, you can't look directly at your finger. It's just too thick for light from underneath to pass through, to reach the microscope lenses. It might be possible to scrape off thin layers of dead skin and put them on a slide to view through a microscope, though.
tutti u kuthi raam da
Staining is done to help determine what the sample is. It makes it visible under a microscope and it gives some information about the structure of the cell. A positive Gram stain means that the cell has a thick peptidoglycan layer.