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For what are FFPE samples used for?

Updated: 9/27/2023
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jonathan.rohr92

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9mo ago

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FFPE human tissue samples are used in medical and scientific research, especially histopathology, as they allow pathologists to diagnose diseases and identify abnormal cellular structures. FFPE samples are also used in immunohistochemistry and in situ hybridization techniques to detect biomarkers, gene expression patterns and to locate proteins. They are also used for genomic studies, transcriptomics analysis and proteomics research. FFPE tissue samples play an important role in drug development, personalized medicine, and biomarker discovery.

We offer human tissue samples on our website and gathered more information and FAQs there, feel free to dive deeper in the topic:

centralbiohub.de/biospecimens/cancer-samples/ffpe-samples

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s.nikam

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9mo ago
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Q: For what are FFPE samples used for?
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How to prepare FFPE samples?

FFPE (Formalin-Fixed Paraffin-Embedded) preparation includes a series of steps. First, the fresh collected tissue is immersed in a formaldehyde (formalin) solution to fix the cellular structures. This fixed tissue is then dehydrated with a series of alcohol dilutions and cleared by using a clearing agent. After that, the tissue is embedded in liquid paraffin wax. Finally, after the wax solified, the tissue wax block is sectioned into thin slices using a microtome. For further analysis, these since slices can be mounted onto a coverglass. To learn more about FFPE samples, I recommend to check out the FAQ on our website where we offer human tissue samples: centralbiohub.de/biospecimens/cancer-samples/ffpe-samples


How can you store tissue samples?

To store tissue samples for research, they are most of the time converted into FFPE blocks and stored in an airtight container, preferably in the refrigerator at 40 °C. It is also possible to store them as fresh frozen samples at -80 °C using dry ice or liquid nitrogen tanks. If you want to dive deeper into the topic, you could visit our website, where we offer human tissue samples: centralbiohub.de/biospecimens/tissue-bank/human-tissue-samples


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