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FFPE (Formalin-Fixed Paraffin-Embedded) preparation includes a series of steps. First, the fresh collected tissue is immersed in a formaldehyde (formalin) solution to fix the cellular structures. This fixed tissue is then dehydrated with a series of alcohol dilutions and cleared by using a clearing agent. After that, the tissue is embedded in liquid paraffin wax. Finally, after the wax solified, the tissue wax block is sectioned into thin slices using a microtome. For further analysis, these since slices can be mounted onto a coverglass.

To learn more about FFPE samples, I recommend to check out the FAQ on our website where we offer human tissue samples:

centralbiohub.de/biospecimens/cancer-samples/ffpe-samples

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s.nikam

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1y ago

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For what are FFPE samples used for?

FFPE human tissue samples are used in medical and scientific research, especially histopathology, as they allow pathologists to diagnose diseases and identify abnormal cellular structures. FFPE samples are also used in immunohistochemistry and in situ hybridization techniques to detect biomarkers, gene expression patterns and to locate proteins. They are also used for genomic studies, transcriptomics analysis and proteomics research. FFPE tissue samples play an important role in drug development, personalized medicine, and biomarker discovery. We offer human tissue samples on our website and gathered more information and FAQs there, feel free to dive deeper in the topic: centralbiohub.de/biospecimens/cancer-samples/ffpe-samples


How do you prepare Schultz Reagent?

To prepare Schultz reagent, mix one part of concentrated sulfuric acid with nine parts distilled water. Allow the mixture to cool before use. This reagent is commonly used for the detection of proteins in biological samples.


Why samples should be crushed in experiments?

Samples are crushed in experiments to increase their surface area, making it easier for reactants to come into contact and speeding up reactions. Crushing also helps to ensure homogeneity in the sample, reducing the risk of sampling errors and improving the consistency of results. Additionally, crushed samples are often easier to handle and prepare for analysis.


What is a breaker used for in chemistry?

In chemistry, a breaker refers to a substance that is added to a reaction mixture to stop or inhibit the reaction. Breakers are used to control the reaction kinetics and prevent unwanted side reactions from occurring. Examples include quenching agents, acid catalysts, and inhibitors.


What is the procedure to prepare anthrone reagent?

To prepare anthrone reagent, dissolve 200 mg of anthrone in 100 ml of concentrated sulfuric acid. Mix the solution thoroughly and allow it to cool before use. Anthrone reagent is used for the detection of carbohydrates, specifically sugars, in various biological samples.

Related Questions

For what are FFPE samples used for?

FFPE human tissue samples are used in medical and scientific research, especially histopathology, as they allow pathologists to diagnose diseases and identify abnormal cellular structures. FFPE samples are also used in immunohistochemistry and in situ hybridization techniques to detect biomarkers, gene expression patterns and to locate proteins. They are also used for genomic studies, transcriptomics analysis and proteomics research. FFPE tissue samples play an important role in drug development, personalized medicine, and biomarker discovery. We offer human tissue samples on our website and gathered more information and FAQs there, feel free to dive deeper in the topic: centralbiohub.de/biospecimens/cancer-samples/ffpe-samples


How can you store tissue samples?

To store tissue samples for research, they are most of the time converted into FFPE blocks and stored in an airtight container, preferably in the refrigerator at 40 °C. It is also possible to store them as fresh frozen samples at -80 °C using dry ice or liquid nitrogen tanks. If you want to dive deeper into the topic, you could visit our website, where we offer human tissue samples: centralbiohub.de/biospecimens/tissue-bank/human-tissue-samples


What is histology technician certification?

A histologist will actually prepare tissue samples and other body samples for testing in the laboratory. The certification will come from the American Society of Pathologists.


Where do we usually use micropippetes?

For example pipettes are used in analytical chemistry to prepare samples for analysis or standard solutions.


What does histologist mean?

A histologist is a scientist who specializes in the study of the microscopic structure of tissues. They use various techniques to prepare tissue samples for analysis and help diagnose diseases based on tissue samples.


What does a cytologist use?

They use:light microscopesfluorescence microscopesmicroscope slidescervical spatulas for PAP smearsbronchoscopes for bronchial brushingssyringes and needles for needle aspiration biopsiesstaining tools to stain samples prior to viewingprobe preparation equipment to prepare samples for Fluorescence in situ hybridization (FISH)


How do you prepare Schultz Reagent?

To prepare Schultz reagent, mix one part of concentrated sulfuric acid with nine parts distilled water. Allow the mixture to cool before use. This reagent is commonly used for the detection of proteins in biological samples.


Why samples should be crushed in experiments?

Samples are crushed in experiments to increase their surface area, making it easier for reactants to come into contact and speeding up reactions. Crushing also helps to ensure homogeneity in the sample, reducing the risk of sampling errors and improving the consistency of results. Additionally, crushed samples are often easier to handle and prepare for analysis.


Do i say can i have samples about them or samples of them?

samples of them


What is cancer and the cause of cancer?

Cancer is a medical condition characterised by the abnormal and uncontrolled proliferation of cells, resulting in the formation of solid tumours that can spread to healthy tissues and create neoplasms. Central BioHub offers a vast collection of cancer samples such as ffpe tumour tissue blocks, frozen tissue blocks, serum, plasma, and urine samples screened for cancer-antigen 19-9 (CA 19-9), cancer-antigen 125 (CA 125), carcinoembryonic antigen (CEA), and prostate-specific antigen (PSA). The samples are gathered from patients at various stages of cancer and are available with a complete clinical evaluation profile. Hurry up, order richly characterised cancer samples online from Central BioHub


How must samples be prepare for observation by electron microscope?

They must be preserved and dehydrated. Once this is accomplished, put the specimen onto a slide, and then the slide is ready to be viewed under the microscope.


Where can I find samples of respiratory therapist tests?

You could check out www.studystack.com, www.testprepreview.com, and also YouTube. I found many sample tests and questions that would help you prepare for your tests.