0
chelating Mg2+
Wiki User
∙ 10y ago
Add your answer:
Earn +20 pts
What is the function of 2-propanol in DNA extraction?
To achieve precipitation DNA.
What is the function of chloroform in DNA extraction?
It solubalize lipids and protiens to remove them from DNA
What is the function of dithiothreitol in DNA extraction?
DTT is the reducing agent for thiolated DNA
What is the function of CL buffer in DNA extraction?
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
What is the function of ascorbic acid in DNA extraction?
removal of polyphenols
What is the function of ammonium acetate in DNA extraction?
ammonium acetae use to percipitate DNA from water.
Function chloroform in extraction DNA from blood?
to remove excess phenol from DNA to remove excess phenol from DNA
What is the function of tritonx100 in DNA extraction?
Triton X-100 is used as a lysis buffer for DNA separation.
What is the function of salt in DNA extraction?
salt binds to molecules to keep it from clumping
What is function of Buffer AP1 in DNA extraction?
The buffer AP1 is vital in DNA extraction as it acts as a cleanser to break up the lipids surrounding the cellular membrane. The buffer also maintains the right environment for the DNA so it is not damaged during the extraction process.
Role of te in dna extraction?
TE stands for Tris and EDTA. The Tris buffers the water to prevent acid hydrolysis of the DNA/RNA. The EDTA chelates divalent cations that can assist in the degradation of RNA.
What is the function of ethidium bromide in DNA extraction?
EtBr interculates into DNA and when exposed to UV light, it causeses DNA to nick and therefore uncoil
