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The process of gram staining is simple.
1)smear bacteria from pure culture onto slide, heat fix
2)flood with crystal violet (1min)
3)Add iodine (1 min)
4)acid/alcohol wash (1 min)
5)Flood with safranine (1min)
6)Air dry and examine.
These times are for clinical microbiology and experimental methods employ optimal and more precise times (but overall its pretty close).
Down side of this method is that you must smear bacteria onto the slide and fix it by heating the underside of the slide with a bunsen burner. if they are pink then you have gram negative (Gram's stain didnt stick) if its purple then its gram positive(Gram's stain did stick) This is due to the peptidoglycan layers. Gram negative bacteria have only a thin layer of peptidoglycan as part of the cell membrane/wall where Gram positive have a very think peptidoglycan layer.
Source(s):
Medical Microbiology
What else can I help you with?
Is azospirillum gram positive or gramnegative?
Azospirillum is a Gram-negative bacterium.
Why is important to distinguish between Gram-positive and Gram-negative bacteria in diagnosing a bacterial infection?
gram- negative and gram- positive bacteria differ in their response to different antibiotics
What method is used to distinguish the two types of bacteria?
One common method to distinguish between different types of bacteria is Gram staining. This method involves staining bacteria with crystal violet and iodine, followed by a decolorization step and counterstain. Based on whether bacteria retain the stain (Gram-positive) or not (Gram-negative), they can be differentiated.
What stain is used to distinguish differences between the cell walls of medically important bacteria?
Gram stain is commonly used to distinguish differences between the cell walls of medically important bacteria. This technique categorizes bacteria into Gram-positive (purple) and Gram-negative (pink) based on their cell wall structure, aiding in identification and classification of bacteria in microbiology.
What kingdom is gram positive bacteria?
Kingdom Gram-Positive Bacteria is a kingdom within the domain Bacteria.
Does Gram-positive bacteria have lipopolysaccharides?
No, Gram-positive bacteria do not have lipopolysaccharides.
Is lactobacillus bacteria gram positive bactaria?
Lactobacillus is gram positive bacteria
Bacteria are classified as either gram positive or gram negative based on their ability to absorb gentian violet dye into their cell walls True or False?
True. Gram staining is a common technique used to distinguish between different groups of bacteria based on differences in their cell wall structure. Gram positive bacteria retain the violet dye and appear purple, while gram negative bacteria do not retain the dye and appear pink.
Why are bacteria divided into two domains?
Bacteria are divided into two domains, Archaea and Bacteria, based on genetic and biochemical differences. Archaea have unique characteristics that distinguish them from traditional bacteria, such as operating in extreme environments. This separation helps scientists classify and study these organisms more effectively.
Is azospirillum a gram positive bacteria?
Gram positive
Do gram positive bacteria have an outer membrane?
No, gram positive bacteria do not have an outer membrane.
Do gram positive bacteria have lipopolysaccharides (LPS)?
No, gram positive bacteria do not have lipopolysaccharides (LPS).
