To prepare a 1 micro mole solution of phenylephrine in 1 mL of distilled water, you will need to know the molar mass of phenylephrine and use it to calculate the required amount of phenylephrine powder. Here are the steps to prepare the solution:
Determine the molar mass of phenylephrine: The molar mass of phenylephrine is 203.28 g/mol.
Calculate the mass of phenylephrine needed: Since you want a 1 micro mole solution, which is equivalent to 1 x 10^-6 moles, you can use the formula:
Mass (g) = Moles x Molar mass
Mass (g) = 1 x 10^-6 moles x 203.28 g/mol
Mass (g) = 0.00020328 g
Therefore, you will need 0.00020328 grams of phenylephrine.
Weigh out the required amount of phenylephrine: Use a digital balance to accurately measure 0.00020328 grams of phenylephrine powder.
Transfer the phenylephrine powder to a clean and dry container, such as a vial or test tube.
Add 1 mL of distilled water to the container containing the phenylephrine powder. Ensure that the water completely dissolves the powder by gently swirling or stirring the solution.
Once the phenylephrine is completely dissolved, your 1 micro mole solution of phenylephrine in 1 mL of distilled water is ready for use.
Note: It is important to handle chemicals and measurements with care and follow proper laboratory safety protocols while preparing solutions.