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How Pulsed-Field Gel Electrophoresis differences from PCR identification of bacterial strain?
Wiki User
∙ 10y ago
In using PCR to identify a bacterial strain, a single segment of DNA is amplified, typically an rRNA gene or a portion of it. The amplified fragment is then sequenced and the sequence compared to that of sequences in the databases. Since rRNA gene sequences are so conserved, this method does not have the resolution to distinguish individual strains of the same species, although it can be used for species identification.
In PFGE, there is no amplification. Instead, restriction enzymes are used to digest chromosomal DNA to generate a characteristic pattern. Also, the detection method is staining of the DNA, not DNA sequencing
Wiki User
∙ 10y ago
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