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You have performed a transformation experiment using a mutant strain that is arg-- trp+ gal-- his--. A culture of this mutant strain was mixed with a DNA from another mutant strain that is arg+ trp-- gal+ his+.
How will you select for the following recombinants?
a) arg+ trp+ gal-- his--
b) arg+ trp-- gal+ his+
c) arg-- tryp-- gal+ his--
d) arg-- trp+ gal-- his+
What else can I help you with?
How is recombinant replicated?
Recombinant DNA is replicated using host cells, typically bacteria or yeast, that have been engineered to contain the desired DNA sequence. These host cells are then grown in a lab setting under specific conditions that allow for the replication of the recombinant DNA. The cell division process allows for the production of multiple copies of the recombinant DNA.
What is a collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids called?
A DNA LibraryA collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids is called a DNA library. RNA can manufacture DNA via the action of reverse transcriptase.
How will you identify cells carrying the recombinant plasmid?
The transformants are selected for on agar containing an appropriate antibiotic. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. PCR can then be performed on the colonies to ensure they contain your gene of interest on the plasmid.
What is a molecule containing DNA from two different organism called?
I'm not quite sure what you mean by your question, but if you are asking about what organisms contain the DNA of another organism, the human body could count as one. The mitochondria within our cells have a DNA that is different from our own DNA strands, which caused many scientists to believe that they might have once been another organism all-together. They replicate using their own processes, too. Hope that helps!
What step is not essential in producing recombinant DNA?
One non-essential step in producing recombinant DNA is incorporating a selection marker gene. While this can be useful for identifying cells that have successfully taken up the recombinant DNA, it is not absolutely necessary for the process of creating recombinant DNA itself.
What is a DNA molecule containing regions from different sources?
A DNA molecule containing regions from different sources is called recombinant DNA. This is often created in laboratories by combining DNA from different organisms or through genetic engineering techniques. Recombinant DNA technology has many applications in biotechnology and genetic research.
How is recombinant replicated?
Recombinant DNA is replicated using host cells, typically bacteria or yeast, that have been engineered to contain the desired DNA sequence. These host cells are then grown in a lab setting under specific conditions that allow for the replication of the recombinant DNA. The cell division process allows for the production of multiple copies of the recombinant DNA.
What is a collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids called?
A DNA LibraryA collection of cells containing DNA fragments produced by restriction enzymes and incorporated into plasmids is called a DNA library. RNA can manufacture DNA via the action of reverse transcriptase.
List the four distinct stages involved in a gene transfer?
1.Cleaving DNA The source chromosomes is cut into fragments of DNA.2.Producing recombinant DNA The DNA fragments containing the desired gene are inserted into viral or bacterial DNA. The recombinant DNA is then allowed to infect the target cells.3.Cloning cells infected cells are allowed to reproduce.Growing a large number of identical cells from one cell is known as cloning.4.Screening target cells Targets cells that have received the particular gene of interest are isolated.
How will you identify cells carrying the recombinant plasmid?
The transformants are selected for on agar containing an appropriate antibiotic. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. PCR can then be performed on the colonies to ensure they contain your gene of interest on the plasmid.
What is a molecule containing DNA from two different organism called?
I'm not quite sure what you mean by your question, but if you are asking about what organisms contain the DNA of another organism, the human body could count as one. The mitochondria within our cells have a DNA that is different from our own DNA strands, which caused many scientists to believe that they might have once been another organism all-together. They replicate using their own processes, too. Hope that helps!
DNA molecules that are produced by combining DNA from different sources are called?
The word you're looking for may be "recombinant".
What are the requirements for recombinant DNA technology?
Requirements for recombinant DNA technology include a vector (such as a plasmid or virus) to carry the desired DNA fragment, restriction enzymes to cut the DNA at specific sites, and DNA ligase to join the DNA fragments together. Additionally, cells capable of taking up and expressing the recombinant DNA are needed, along with appropriate selection markers to identify successfully transformed cells.
What step is not essential in producing recombinant DNA?
One non-essential step in producing recombinant DNA is incorporating a selection marker gene. While this can be useful for identifying cells that have successfully taken up the recombinant DNA, it is not absolutely necessary for the process of creating recombinant DNA itself.
Why are bacterial cells useful in recombinant DNA technology?
Bacterial cells are useful in recombinant DNA technology because they can easily take up foreign DNA through a process called transformation. Once the foreign DNA is inserted into a bacterial cell, it can be replicated and amplified quickly. Bacteria are also easy to culture and manipulate in the laboratory, making them ideal for producing large quantities of recombinant proteins or DNA fragments.
What is pcDNA?
pcDNA is DNA vector used to clone recombinant DNA sequences for the expression of proteins of interest in mammalian cells.
Splicing DNA from two different organisms produces a new DNA segment called?
When DNA contains parts from two or more organisms it is recombined. Recombinant DNA is often used in genetic engineering. A natural process of DNA recombination is called sexual reproduction.
