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bacterial plasmid/ plasmid ring
I think I know the answer... it's 5
a human cells have DNA and bacteria has plasmid
Bacteria generally have one (and only one) circular chromosome. However, they can also contain smaller rings of DNA called plasmids. These plasmids are generally not essential to cellular functions, but serve a variety of purposes. For example, they can serve as cloning vectors.
If you transform bacteria with a plasmid containing a selection marker (such as an antibiotic resistance gene) and plate the transformed bacteria on a plate suited for selecting for plasmid-containing bacteria (such as a plate containing an antibiotic that only those bacteria with antibiotic resistance can survive), then simply inspecting whether colonies are present on the plate will suffice in determining whether the transformation succeeded. If no colonies are found, that means no bacteria got the antibiotic resistance gene on the plasmid and the transformation was unsuccessful. If some colonies are found, that means some bacteria contain the plamis containing the antibiotic resistance gene and those colonies can the transformation was successful.
Organelles inside bacteria do not 'make' GFP. GFP can however be expressed in bacteria by adding the coding sequence for GFP to their genome. This can be done by using a plasmid virus containing the GFP gene sequence. When combined with the plasmid, a very small number of bacteria will take up the plasmid's genome into their own. These bacteria can be isolated by using the 'transform or die' mechanism. Bacteria that do not contain the plasmid are preferentially killed by adding an antibody to the agar. The normal bacteria will not be resistant to this, and will die, but the bacteria which have taken up the plasmid into their genome will also contain an antibody resistance gene. This allows them to live in the presence of the antibody. Since GFP was also added to these bacteria's genome, they will fluoresce.
Plasmid is the property of prokaryotic cell i.e. of bacteria generally no mamalian cell have plasmid but ya there can be chances to have lillte part of DNA sequence to be simillar that of plasmid DNA sequence in prokaryotes
Bacteria
A plasmid (free bit of DNA) in bacteria that produces colicin to kill other strains of bacteria.
If you do not give antibiotics, the plasmid may lost after a few generation. it is because bacteria thinks that the extra DNA (plasmid) is not needed as the antibiotic is not present. That is the reason for keeping the culture always in antibiotic selection pressure.
the plasmid contains a certain gene, which codes for the "Green Flourescent Protein." So you put the plasmid in the bacteria, the plasmid starts making that protein in the bacteria, and boom you've got glowing bacteria. works for bunnies and monkeys too, apparently =)
A plasmid is an extra-chromosomal element. Strictly speaking, it is not a part of the bacterial mail genome. Plasmids, when first discovered in bacteria, were observed to contain genes that confer special features to the bacteria (like drug resistance). Also, a plasmid is capable of autonomous replication, which means it has its own origin of replication and does not have to wait for the bacterial genome to be replicated. When a foreign gene is inserted into a plasmid, the plasmid has to be transformed into the bacteria. Once transformed, the cDNA in the plasmid is able to replicate every time the plasmid replicated. This does not have any affect on the normal functioning of the bacteria. The organism is merely being used to make copes of the foreign gene and/or harvest an important protein product.
Yes
bacterial plasmid/ plasmid ring
Plasmids have many different functions. They may contain genes that enhance the survival of an organism, either by killing other organisms or by defending the host cell by producing toxins. Some plasmids facilitate the process of replication in bacteria.
That is known as a plasmid. The plasmid originally came from outside of the bacteria and was incorporated into the bacteria. Usually, these plasmids are beneficial to the bacteria that takes them in.
Bacteria possess extra chromosomal DNA,called plasmids. Often it carries functional genes for the resistance of bacteria (example: Aromotic compound degrading genes). Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin or acridine orange!