How can micro organisms be grown in a laboratory?

Answer 1

microorganisms can be grown in laboratory by using suitable medias and optimum condition.

MEDIA;it is a artificial arrangement or a material that which allows growth and multiplication of microbe by providing suitable or optimum conditions.generally various types of microbe requires various types of media.

generally most of the microbe, we studied are pathogenic.hence to grow an microbe require ideal conditions that are present in our body and tissue fluids.

but no single media satisfies all essential conditions.most of media are useful up to certain level.

generally these microbes utilize the food materials either by oxidation or reduction and obtain energy and grow and multiply.generally media should contain carbohydrate source,proteins for synthesis of nucleic acids,growth factors etc.

ex of some medias are;agar-agar,liquid broth,mac-conkey agar media,blood agar media,lactose broth media,media containing 1% crystal violet ,media containing bile salts etc.thus these are also used for identification and study various types of nutritional requirements of microbes also.

OPTIMUM CONDITIONS;it include optimum temperature and pH.

optimum temperature required by various microbes differ from each other.

for ex;for bacteria temp required is 37 degrees centigrade.

and for fungi they require room temperature.thus temp differ for various microbes.

VARIOUS INSTRUMENTS ARE AVAILABLE SUCH AS INCUBATOR, FOR MAINTAINING OPTIMUM TEMPERATURES.

optimum pH also varies from each.for bacteria optimum pH is 7.3.some acidophilic can grow at acidic conditions ex;lactic acid bacteria.and basophilic bacteria grow at basic conditions,ex;vibrio cholera.

bacteria can grow at high sugar concentrations and fungi grow at low sugar con.

THUS MOST OF THE MICROBES CAN BE GROWN IN LABORATORY BY PROVIDING SUITABLE OPTIMUM CONDITIONS AND GROWTH MEDIA FOR THEIR GROWTH AND MULTIPLICATION.